Detailed information of TA system TA180209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2544163..2544809
• Replicon: chromosome
• Accession: NZ_CP064118
• Organism: Yersinia pestis strain C-783

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3G5L3F2
• Locus tag: ISU55_RS11670
• Protein ID: WP_002214976.1
• Coordinates: 2544163..2544345 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0U1R391
• Locus tag: ISU55_RS11675
• Protein ID: WP_002211129.1
• Coordinates: 2544390..2544809 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU55_RS11635	2539549..2540019	+	471	WP_002227961.1	flagellar basal body-associated protein FliL	-
ISU55_RS11640	2540025..2541026	+	1002	WP_002211135.1	flagellar motor switch protein FliM	-
ISU55_RS11645	2541023..2541439	+	417	WP_002211134.1	flagellar motor switch protein FliN	-
ISU55_RS11650	2541439..2541975	+	537	WP_002211133.1	flagellar biosynthetic protein FliO	-
ISU55_RS11655	2542056..2542796	+	741	WP_002220494.1	flagellar type III secretion system pore protein FliP	-
ISU55_RS11660	2542902..2543171	+	270	WP_002211131.1	flagellar biosynthesis protein FliQ	-
ISU55_RS11665	2543174..2543956	+	783	WP_002211130.1	flagellar type III secretion system protein FliR	-
ISU55_RS11670	2544163..2544345	+	183	WP_002214976.1	type II toxin-antitoxin system HicA family toxin	Toxin
ISU55_RS11675	2544390..2544809	+	420	WP_002211129.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ISU55_RS11680	2545067..2546026	-	960	WP_002223882.1	sugar kinase	-
ISU55_RS11685	2546075..2547097	-	1023	WP_002214974.1	ABC transporter permease	-
ISU55_RS11690	2547100..2548677	-	1578	WP_002211126.1	sugar ABC transporter ATP-binding protein	-
ISU55_RS11695	2548743..2549738	-	996	WP_002211125.1	substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fliZ / fliA / fliD / fliS / fliT / fliE / fliF / fliG / fliH / fliI / fliJ / fliL / fliM / fliN / fliP / fliQ / fliR / flgL / flgK / flgJ / flgI / flgH / flgG / flgF / flgE / flgD / flgC / flgB / flgA / flgM / flgN / flhE / flhA / flhB	2458561..2580259	121698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-131)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6783.99 Da
• Isoelectric Point: 10.8877

>T180209 WP_002214976.1 NZ_CP064118:2544163-2544345 [Yersinia pestis]
VKSSELIKLLETNGWKLMRVKGSHHQFKHPDFAAVITVPHPKKDVKPGTLRQILKDANLS

Nucleotide

• Length: 183 bp

>T180209 NZ_CP085088:c1784565-1784308 [Streptococcus suis]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAGATTATCCAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCCTGGTCTCGGAGAATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15549.42 Da
• Isoelectric Point: 4.7731

>AT180209 WP_002211129.1 NZ_CP064118:2544390-2544809 [Yersinia pestis]
MAIYPAYVHVDNDGSASGYFPDVKGCIFAINAGEDLFAEASSALDAHFEALVSEGIEIPEAHDMPYHVYRNPCDYADGGQ
WYNVNIDMSKYDGKVERINVTLPHRLIHQIDTIVKVRPEYASRSNFLAEAARKELQKLA

Nucleotide

• Length: 420 bp

>AT180209 NZ_CP085088:c1784827-1784567 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTCCTACGTGGTATGGCTAAAGTCAAAACGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA

Structures

Toxin

• AlphaFold DB: A0A3G5L3F2

Antitoxin

• AlphaFold DB: A0A0U1R391