Detailed information of TA system TA180191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 7589..8386
• Replicon: plasmid unnamed1
• Accession: NZ_CP064114
• Organism: Enterobacter hormaechei strain IR5283

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: ISU86_RS21685
• Protein ID: WP_172754447.1
• Coordinates: 7589..8092 (-)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: ISU86_RS21690
• Protein ID: WP_016582626.1
• Coordinates: 8117..8386 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU86_RS21660	5352..6047	+	696	WP_206807831.1	receptor-recognizing protein	-
ISU86_RS21665	6122..6445	+	324	WP_000064173.1	hypothetical protein	-
ISU86_RS21670	6459..7151	+	693	WP_006812500.1	membrane protein	-
ISU86_RS21675	7153..7404	+	252	WP_000147960.1	hypothetical protein	-
ISU86_RS21680	7355..7561	+	207	WP_080399007.1	hypothetical protein	-
ISU86_RS21685	7589..8092	-	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
ISU86_RS21690	8117..8386	-	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
ISU86_RS21695	8803..9468	+	666	WP_023315962.1	AAA family ATPase	-
ISU86_RS21700	9474..9827	+	354	WP_160956455.1	hypothetical protein	-
ISU86_RS21705	9869..10666	-	798	WP_077990606.1	DUF2971 domain-containing protein	-
ISU86_RS21710	10930..11655	+	726	WP_058652463.1	hypothetical protein	-
ISU86_RS21715	11716..13056	+	1341	WP_006812582.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B	-	1..119573	119573

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T180191 WP_172754447.1 NZ_CP064114:c8092-7589 [Enterobacter hormaechei]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T180191 NZ_CP085085:c1994452-1994195 [Streptococcus suis]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAGATTATCAAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCCTGGTCTCGGAGAATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT180191 WP_016582626.1 NZ_CP064114:c8386-8117 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT180191 NZ_CP085085:c1994714-1994454 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTCCTACGTGGTATGGCTAAAGTCAAAACGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3