Detailed information of TA system TA180179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1059263..1059787
• Replicon: chromosome
• Accession: NZ_CP064113
• Organism: Enterobacter hormaechei strain IR5283

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A837FFM2
• Locus tag: ISU86_RS05060
• Protein ID: WP_015571250.1
• Coordinates: 1059263..1059481 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: ISU86_RS05065
• Protein ID: WP_206807822.1
• Coordinates: 1059509..1059787 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU86_RS05030	1055275..1055535	+	261	WP_015571255.1	type B 50S ribosomal protein L31	-
ISU86_RS05035	1055538..1055678	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
ISU86_RS05040	1055675..1056385	-	711	WP_022650493.1	GNAT family N-acetyltransferase	-
ISU86_RS05045	1056487..1057947	+	1461	WP_058652690.1	PLP-dependent aminotransferase family protein	-
ISU86_RS05050	1057919..1058386	-	468	WP_022650495.1	hypothetical protein	-
ISU86_RS05055	1058503..1059054	-	552	WP_022650496.1	maltose O-acetyltransferase	-
ISU86_RS05060	1059263..1059481	-	219	WP_015571250.1	hemolysin expression modulator Hha	Toxin
ISU86_RS05065	1059509..1059787	-	279	WP_206807822.1	Hha toxicity modulator TomB	Antitoxin
ISU86_RS05070	1059820..1060517	+	698	Protein_979	IS1 family transposase	-
ISU86_RS05075	1060529..1060660	-	132	Protein_980	Hha toxicity attenuator	-
ISU86_RS05080	1061171..1064317	-	3147	WP_022650497.1	multidrug efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1060014..1060517	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (7-82)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8583.95 Da
• Isoelectric Point: 8.9008

>T180179 WP_015571250.1 NZ_CP064113:c1059481-1059263 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T180179 NZ_CP085082:797033-797380 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10564.01 Da
• Isoelectric Point: 6.9454

>AT180179 WP_206807822.1 NZ_CP064113:c1059787-1059509 [Enterobacter hormaechei]
ISKLAASPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINAQDLQKWRKSGNRLFRCFT
NVSRANPVSLSC

Nucleotide

• Length: 279 bp

>AT180179 NZ_CP085082:796772-797023 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Toxin

• AlphaFold DB: A0A837FFM2