180177

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 18558..19201 Replicon plasmid unnamed3
Accession NZ_CP064111
Organism Klebsiella oxytoca strain IR5392

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag ISU85_RS29630 Protein ID WP_000754566.1
Coordinates 18785..19201 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag ISU85_RS29625 Protein ID WP_001261276.1
Coordinates 18558..18788 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU85_RS29590 13677..13946 + 270 WP_000339857.1 hypothetical protein -
ISU85_RS29595 14123..14989 - 867 WP_004118283.1 replication initiation protein -
ISU85_RS29600 15519..15623 + 105 WP_032409716.1 hypothetical protein -
ISU85_RS29605 15752..16009 + 258 WP_000764642.1 hypothetical protein -
ISU85_RS29610 16067..16843 - 777 WP_000015958.1 site-specific integrase -
ISU85_RS29615 16840..17583 - 744 WP_000129823.1 hypothetical protein -
ISU85_RS29620 17634..17984 - 351 WP_000493378.1 hypothetical protein -
ISU85_RS29625 18558..18788 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ISU85_RS29630 18785..19201 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
ISU85_RS29635 19405..20213 + 809 Protein_22 IS3-like element ISEc15 family transposase -
ISU85_RS29640 20319..21155 - 837 WP_000480968.1 aminoglycoside O-phosphotransferase APH(6)-Id -
ISU85_RS29645 21155..21958 - 804 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
ISU85_RS29655 23259..23873 - 615 WP_000904906.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(6)-Id / aph(3'')-Ib - 1..37970 37970


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T180177 WP_000754566.1 NZ_CP064111:18785-19201 [Klebsiella oxytoca]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T180177 NZ_CP085080:c4873234-4872863 [Enterobacter cloacae]
GTGAACAATATGGCCGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTCGAAGCTGCGGATGCGATTGA
TCATGCCGCGTCACATCGAGCCATTAGCTTAATTACCTGGATGGAGGTGATGGTGGGCGCACGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTCGAAATTATTGATGTGTCTCGGGATATCGCGGAAAGAAGCGTTTTACTGCGT
GAAAAGCATGGAATGAAACTGCCTGATGCCATCATTCTGGCCACAGCCCAGAGCAGAAAATGTCCGTTGATTTCTCGAAA
TACGAAAGATTTTTCGGGTATTGATGAGGTCGTTACGCCTTATCAACTGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT180177 WP_001261276.1 NZ_CP064111:18558-18788 [Klebsiella oxytoca]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT180177 NZ_CP085080:c4873479-4873237 [Enterobacter cloacae]
ATGAGTGTTTTGGCGGAACTGGATATGGGCAGAATACTGATCGATTTATCTGATGACGTGATTCAACGGCTGGATAACCT
CAAGCAACTGCGAAATCAGCCGCGCGCTGAGCTGCTACGGGAGGCGATAGAGCTGTATCTCGACCAGCAGAGTTCGTCTG
TTATCCGCGGTGCGCTTGGCTTGTGGGGAGACAAGAAAGAAGACGGGCTGGAGTATGAACGTAAGCTGCGTGAGGAATGG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References