Detailed information of TA system TA180175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 80854..81764
• Replicon: plasmid unnamed2
• Accession: NZ_CP064110
• Organism: Klebsiella oxytoca strain IR5392

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: ISU85_RS28820
• Protein ID: WP_004181896.1
• Coordinates: 81294..81764 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: ISU85_RS28815
• Protein ID: WP_004181895.1
• Coordinates: 80854..81297 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU85_RS28790	76010..76225	-	216	Protein_81	IS200/IS605 family transposase	-
ISU85_RS28795	76227..76796	+	570	Protein_82	transposase	-
ISU85_RS28800	77487..78974	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
ISU85_RS28805	79060..79482	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
ISU85_RS28810	79482..80753	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
ISU85_RS28815	80854..81297	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
ISU85_RS28820	81294..81764	+	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
ISU85_RS28825	81874..82134	-	261	WP_004026352.1	hypothetical protein	-
ISU85_RS28830	82705..83886	-	1182	WP_004883380.1	IS481 family transposase	-
ISU85_RS28835	83958..84116	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
ISU85_RS28840	84719..85693	+	975	WP_004196336.1	hypothetical protein	-
ISU85_RS28845	85891..86268	-	378	WP_004181901.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..223677	223677
	-	flank	IS/Tn	-	-	76443..76796	353
	-	flank	IS/Tn	-	-	82705..83886	1181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T180175 WP_004181896.1 NZ_CP064110:81294-81764 [Klebsiella oxytoca]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T180175 NZ_CP085080:c4015365-4014952 [Enterobacter cloacae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCGTTATTGCTCCACGGCCTGGTCGCGGC
ATTTGTTTTACTGATGCCGTGGCCGCTTAGCTATACGCCGCTGTGGCTGCTGTTGCTGTCGTTCGTCGTTTTTGACAGCG
TGCGCAGCCAGCGCCGGATCAATGCCCGCCAGGGTGAGATCAAATTACTGATGGACTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATCCTCGGTATGCCCTGGATGCTCAGTTCCGGCATGATGCTACGGTTACGCCAGGTGGGCGGCGGTAAGTG
CCAGCATCTGTGGCTGGCCGCCGACAGTATGGATATTGCGGAGTGGCGGGAACTGCGCCGGATGCTACTGCAGCAGCAAC
CGACGCAGGAATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT180175 WP_004181895.1 NZ_CP064110:80854-81297 [Klebsiella oxytoca]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT180175 NZ_CP085080:c4015612-4015346 [Enterobacter cloacae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCATGCCGACGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGAGGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9