Detailed information of TA system TA180174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 87340..88137
• Replicon: plasmid unnamed1
• Accession: NZ_CP064109
• Organism: Klebsiella oxytoca strain IR5392

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: ISU85_RS28250
• Protein ID: WP_172397437.1
• Coordinates: 87340..87843 (-)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: ISU85_RS28255
• Protein ID: WP_004109769.1
• Coordinates: 87868..88137 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU85_RS28225	84337..85770	+	1434	WP_004109808.1	tail fiber domain-containing protein	-
ISU85_RS28230	85870..86193	+	324	WP_004109805.1	hypothetical protein	-
ISU85_RS28235	86210..86902	+	693	WP_064411604.1	hypothetical protein	-
ISU85_RS28240	86904..87155	+	252	WP_004109798.1	hypothetical protein	-
ISU85_RS28245	87088..87312	+	225	WP_072026924.1	hypothetical protein	-
ISU85_RS28250	87340..87843	-	504	WP_172397437.1	GNAT family N-acetyltransferase	Toxin
ISU85_RS28255	87868..88137	-	270	WP_004109769.1	DUF1778 domain-containing protein	Antitoxin
ISU85_RS28260	88509..89174	+	666	WP_004110195.1	AAA family ATPase	-
ISU85_RS28265	89180..89536	+	357	WP_072196454.1	hypothetical protein	-
ISU85_RS28270	89578..91296	+	1719	WP_146106781.1	hypothetical protein	-
ISU85_RS28275	91469..92194	+	726	WP_098992646.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..111771	111771

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18573.27 Da
• Isoelectric Point: 8.8869

>T180174 WP_172397437.1 NZ_CP064109:c87843-87340 [Klebsiella oxytoca]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFEAE

Nucleotide

• Length: 504 bp

>T180174 NZ_CP085080:c2956769-2956666 [Enterobacter cloacae]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10112.58 Da
• Isoelectric Point: 7.0155

>AT180174 WP_004109769.1 NZ_CP064109:c88137-87868 [Klebsiella oxytoca]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMNAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT180174 NZ_CP085080:2956653-2956804 [Enterobacter cloacae]
CTTTCAATCAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGC
TAAAAGTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT