Detailed information of TA system TA180173

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 5604172..5604851
• Replicon: chromosome
• Accession: NZ_CP064108
• Organism: Klebsiella oxytoca strain IR5392

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: ISU85_RS26365
• Protein ID: WP_098992213.1
• Coordinates: 5604172..5604513 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: ISU85_RS26370
• Protein ID: WP_098992212.1
• Coordinates: 5604534..5604851 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU85_RS26340	5599362..5600393	-	1032	WP_023320262.1	DUF1176 domain-containing protein	-
ISU85_RS26345	5600867..5601571	+	705	WP_032726119.1	DNA/RNA non-specific endonuclease	-
ISU85_RS26350	5601860..5602183	+	324	WP_023320260.1	endoribonuclease SymE	-
ISU85_RS26355	5602330..5602764	+	435	WP_023320259.1	VOC family protein	-
ISU85_RS26360	5603413..5604057	+	645	WP_098992214.1	hypothetical protein	-
ISU85_RS26365	5604172..5604513	-	342	WP_098992213.1	TA system toxin CbtA family protein	Toxin
ISU85_RS26370	5604534..5604851	-	318	WP_098992212.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ISU85_RS26375	5604869..5605090	-	222	WP_098992211.1	DUF987 domain-containing protein	-
ISU85_RS26380	5605099..5605581	-	483	WP_098992210.1	DNA repair protein RadC	-
ISU85_RS26385	5605590..5606048	-	459	WP_098992209.1	antirestriction protein	-
ISU85_RS26390	5606135..5606371	-	237	Protein_5163	DUF905 family protein	-
ISU85_RS26395	5606448..5606858	-	411	WP_016243020.1	hypothetical protein	-
ISU85_RS26400	5606924..5607361	-	438	WP_098992208.1	hypothetical protein	-
ISU85_RS26405	5607403..5607939	-	537	WP_098992207.1	DUF4339 domain-containing protein	-
ISU85_RS26410	5607965..5608675	-	711	WP_098992206.1	DeoR family transcriptional regulator	-
ISU85_RS26415	5608883..5609707	-	825	WP_098992205.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5602330..5634932	32602

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12879.91 Da
• Isoelectric Point: 8.9786

>T180173 WP_098992213.1 NZ_CP064108:c5604513-5604172 [Klebsiella oxytoca]
MKPQPATTSRAVKPCLSPVAVWQMLLTRLLEQHYGLKMNDTPFSDETVIKEHIDAGITLADAVNFLVDKYELVRIDRRGF
SWQEQSPYLRAVDILRARQATGLLRRSLNDAVL

Nucleotide

• Length: 342 bp

>T180173 NZ_CP085080:c1230369-1230007 [Enterobacter cloacae]
ATGTGGAACGTCGAGACAACAGACAGATTTGATGAATGGTTCTTTGAACAAACAACAGCGTTAAAAGAAGATGTGCTGGC
AGCAATGCATATTCTTTCTGAGTTTGGTCCGCAACTTGGGCGGCCGTTTGTAGACACCGTGAAGGCTTCAGCCTTTGGGA
ACATGAAAGAGTTACGAATTCAGCACAGAGGAAATCCCATCAGGGCCTTTTTTGCTTTTGATCCTGACCGCAAAGCAATT
GTGCTCTGTGCCGGGGACAAAACCGGTTGCAACCAAAAACGGTTTTATAAAGAGATGATAGCGCTGGCTGACGTTGAGTA
TCACAGACATCTGGCGAATAAGGAGGCCATATGGCAACGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11805.39 Da
• Isoelectric Point: 6.3130

>AT180173 WP_098992212.1 NZ_CP064108:c5604851-5604534 [Klebsiella oxytoca]
MSNTTWGLQRDITPSLGARLVQEGNRLHYLADRASITGKFSDEECLKLDVVFPHFISQIESMLTTGELNPRHAHCVTLYY
NSFICEADTLGSCGYVHIAIYPAQR

Nucleotide

• Length: 318 bp

>AT180173 NZ_CP085080:c1230019-1229720 [Enterobacter cloacae]
ATGGCAACGCTAAAAGAACTTATGGCACAACAGAGCGCGGAAAGTCAGGAGCGCATTGCGGCGAAAGTTGAAGTAATGCG
CAGAATTGTTGCGCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCCACGGTGGCGAAAATCGAACAGCCGGGAAACGACCCCCGACTTTCAACGCTCAAACGCTATGTGAGTGCGCTTGGA
GGGGAGCTAAGCATCGATGTGACGTTACCAAACGGGAAACGGATAGCGTTTGAGATTTAA