Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 3302205..3303828 Replicon chromosome
Accession NZ_CP064108
Organism Klebsiella oxytoca strain IR5392

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag ISU85_RS15645 Protein ID WP_000088044.1
Coordinates 3302503..3303828 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag ISU85_RS15640 Protein ID WP_012477377.1
Coordinates 3302205..3302498 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU85_RS15615 3297342..3298046 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
ISU85_RS15620 3298103..3298396 + 294 Protein_3041 hypothetical protein -
ISU85_RS15625 3299055..3299711 + 657 WP_000462754.1 trypsin-like serine protease -
ISU85_RS15630 3299914..3300411 + 498 WP_000340139.1 membrane protein -
ISU85_RS15635 3300416..3301804 + 1389 WP_098992326.1 hypothetical protein -
ISU85_RS15640 3302205..3302498 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
ISU85_RS15645 3302503..3303828 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
ISU85_RS15650 3303889..3304095 + 207 WP_000134171.1 hypothetical protein -
ISU85_RS15655 3304196..3304606 + 411 WP_000985909.1 hypothetical protein -
ISU85_RS15660 3304619..3305434 + 816 WP_001641519.1 HNH endonuclease -
ISU85_RS15665 3305687..3306112 + 426 WP_000278471.1 hypothetical protein -
ISU85_RS15670 3306661..3306969 + 309 WP_001224686.1 hypothetical protein -
ISU85_RS15675 3306985..3307206 + 222 Protein_3052 HNH endonuclease -
ISU85_RS15680 3307484..3308488 - 1005 WP_000427619.1 IS110-like element IS5075 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island tet(D) / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / qnrB4 / blaDHA-1 - 3284040..3357270 73230


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T180170 WP_000088044.1 NZ_CP064108:3302503-3303828 [Klebsiella oxytoca]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T180170 NZ_CP085080:556455-556739 [Enterobacter cloacae]
ATGACTTATGAACTGGAATTTGACCCGAGGGCGTTAAAAGAGTGGAGCAAGCTGGGGGAGACTGTAAAAAAGCAGTTCAG
GAAGAAACTTGCCGGGGTGTTGGTAAACCCCCGGATCGAATCGGCCCGTCTGCATAACCTGCCTGATTGCTACAAAATAA
AACTCAGATCCCAGGGTTACCGTTTGGTCTATCAGGTTCAGGACAACGTGGTAACGGTAGTTGTCATCGCCACTGGGAAA
AGAGAGAAATCAGCCGTATATCACGATGCTAACAAACGGCTATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT180170 WP_012477377.1 NZ_CP064108:3302205-3302498 [Klebsiella oxytoca]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT180170 NZ_CP085080:556223-556465 [Enterobacter cloacae]
ATGGCTACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACAGAAGCCGTGCGACTCCTTTTTCAGTACGTTGCGGAGACGGGACGCATGCCGGTGAAAACGGTCACGATTAGCGACA
GCGAAGATGCGCTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATTAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References