Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 69856..70499 Replicon plasmid unnamed1
Accession NZ_CP064104
Organism Escherichia coli strain IR53017

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag ISU84_RS26000 Protein ID WP_001034044.1
Coordinates 70083..70499 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag ISU84_RS25995 Protein ID WP_001261286.1
Coordinates 69856..70086 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU84_RS25975 66487..67242 - 756 WP_000852146.1 replication initiation protein RepE -
ISU84_RS25980 67964..68770 - 807 WP_000016970.1 site-specific integrase -
ISU84_RS25985 68771..69076 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
ISU84_RS25990 69078..69296 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
ISU84_RS25995 69856..70086 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ISU84_RS26000 70083..70499 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
ISU84_RS26005 70574..72139 + 1566 WP_001128474.1 AAA family ATPase -
ISU84_RS26010 72124..73146 + 1023 WP_000361402.1 helicase UvrD -
ISU84_RS26015 73400..74086 - 687 Protein_94 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / aadA5 / qacE / sul1 / mph(A) / tet(B) iutA / iucD / iucC / iucB / iucA 1..145347 145347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T180158 WP_001034044.1 NZ_CP064104:70083-70499 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T180158 NZ_CP085074:3845464-3845742 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT180158 WP_001261286.1 NZ_CP064104:69856-70086 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT180158 NZ_CP085074:3845201-3845461 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References