180157

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 68771..69296 Replicon plasmid unnamed1
Accession NZ_CP064104
Organism Escherichia coli strain IR53017

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag ISU84_RS25985 Protein ID WP_001159871.1
Coordinates 68771..69076 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag ISU84_RS25990 Protein ID WP_000813630.1
Coordinates 69078..69296 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU84_RS25970 64733..65899 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
ISU84_RS25975 66487..67242 - 756 WP_000852146.1 replication initiation protein RepE -
ISU84_RS25980 67964..68770 - 807 WP_000016970.1 site-specific integrase -
ISU84_RS25985 68771..69076 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
ISU84_RS25990 69078..69296 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ISU84_RS25995 69856..70086 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ISU84_RS26000 70083..70499 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
ISU84_RS26005 70574..72139 + 1566 WP_001128474.1 AAA family ATPase -
ISU84_RS26010 72124..73146 + 1023 WP_000361402.1 helicase UvrD -
ISU84_RS26015 73400..74086 - 687 Protein_94 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / aadA5 / qacE / sul1 / mph(A) / tet(B) iutA / iucD / iucC / iucB / iucA 1..145347 145347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T180157 WP_001159871.1 NZ_CP064104:c69076-68771 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T180157 NZ_CP085074:c3839402-3839004 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT180157 WP_000813630.1 NZ_CP064104:c69296-69078 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT180157 NZ_CP085074:c3839698-3839405 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References