Detailed information of TA system TA180145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4624949..4625544
• Replicon: chromosome
• Accession: NZ_CP064103
• Organism: Escherichia coli strain IR53017

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: ISU84_RS22340
• Protein ID: WP_000239581.1
• Coordinates: 4624949..4625299 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: ISU84_RS22345
• Protein ID: WP_001223213.1
• Coordinates: 4625293..4625544 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU84_RS22320	4620403..4621425	-	1023	WP_001298067.1	ABC transporter permease	-
ISU84_RS22325	4621439..4622941	-	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
ISU84_RS22330	4623074..4624030	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
ISU84_RS22335	4624340..4624870	+	531	WP_000055075.1	inorganic diphosphatase	-
ISU84_RS22340	4624949..4625299	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
ISU84_RS22345	4625293..4625544	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
ISU84_RS22350	4625756..4626097	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
ISU84_RS22355	4626100..4629879	-	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T180145 WP_000239581.1 NZ_CP064103:c4625299-4624949 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T180145 NZ_CP085074:c836922-836548 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT180145 WP_001223213.1 NZ_CP064103:c4625544-4625293 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT180145 NZ_CP085074:c837380-837012 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7