Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3887378..3887996 Replicon chromosome
Accession NZ_CP064103
Organism Escherichia coli strain IR53017

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ISU84_RS18735 Protein ID WP_001291435.1
Coordinates 3887778..3887996 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ISU84_RS18730 Protein ID WP_000344800.1
Coordinates 3887378..3887752 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU84_RS18720 3882467..3883660 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ISU84_RS18725 3883683..3886832 + 3150 WP_207017292.1 multidrug efflux RND transporter permease subunit AcrB -
ISU84_RS18730 3887378..3887752 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ISU84_RS18735 3887778..3887996 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ISU84_RS18740 3888168..3888719 + 552 WP_000102574.1 maltose O-acetyltransferase -
ISU84_RS18745 3888835..3889305 + 471 WP_022645280.1 YlaC family protein -
ISU84_RS18750 3889469..3891019 + 1551 WP_022645279.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ISU84_RS18755 3891061..3891414 - 354 WP_000878140.1 DUF1428 domain-containing protein -
ISU84_RS18765 3891793..3892104 + 312 WP_000409911.1 MGMT family protein -
ISU84_RS18770 3892135..3892707 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T180141 WP_001291435.1 NZ_CP064103:3887778-3887996 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T180141 NZ_CP085074:226540-226647 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT180141 WP_000344800.1 NZ_CP064103:3887378-3887752 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT180141 NZ_CP085074:c226492-226426 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References