Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2764752..2765390 Replicon chromosome
Accession NZ_CP064103
Organism Escherichia coli strain IR53017

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ISU84_RS13180 Protein ID WP_000813794.1
Coordinates 2765214..2765390 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ISU84_RS13175 Protein ID WP_001270286.1
Coordinates 2764752..2765168 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU84_RS13155 2759904..2760845 - 942 WP_022645667.1 ABC transporter permease -
ISU84_RS13160 2760846..2761859 - 1014 WP_022645666.1 ABC transporter ATP-binding protein -
ISU84_RS13165 2761877..2763022 - 1146 WP_000047466.1 ABC transporter substrate-binding protein -
ISU84_RS13170 2763267..2764673 - 1407 WP_022645665.1 PLP-dependent aminotransferase family protein -
ISU84_RS13175 2764752..2765168 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ISU84_RS13180 2765214..2765390 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ISU84_RS13185 2765612..2765842 + 231 WP_000491567.1 DUF2554 family protein -
ISU84_RS13190 2765934..2767895 - 1962 WP_023909195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ISU84_RS13195 2767968..2768504 - 537 WP_000429148.1 DNA-binding transcriptional regulator SutR -
ISU84_RS13200 2768557..2769768 + 1212 WP_071788120.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T180137 WP_000813794.1 NZ_CP064103:c2765390-2765214 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T180137 NZ_CP085070:c133656-133351 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT180137 WP_001270286.1 NZ_CP064103:c2765168-2764752 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT180137 NZ_CP085070:c133876-133658 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References