Detailed information of TA system TA180137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2764752..2765390
• Replicon: chromosome
• Accession: NZ_CP064103
• Organism: Escherichia coli strain IR53017

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ISU84_RS13180
• Protein ID: WP_000813794.1
• Coordinates: 2765214..2765390 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ISU84_RS13175
• Protein ID: WP_001270286.1
• Coordinates: 2764752..2765168 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU84_RS13155	2759904..2760845	-	942	WP_022645667.1	ABC transporter permease	-
ISU84_RS13160	2760846..2761859	-	1014	WP_022645666.1	ABC transporter ATP-binding protein	-
ISU84_RS13165	2761877..2763022	-	1146	WP_000047466.1	ABC transporter substrate-binding protein	-
ISU84_RS13170	2763267..2764673	-	1407	WP_022645665.1	PLP-dependent aminotransferase family protein	-
ISU84_RS13175	2764752..2765168	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ISU84_RS13180	2765214..2765390	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ISU84_RS13185	2765612..2765842	+	231	WP_000491567.1	DUF2554 family protein	-
ISU84_RS13190	2765934..2767895	-	1962	WP_023909195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ISU84_RS13195	2767968..2768504	-	537	WP_000429148.1	DNA-binding transcriptional regulator SutR	-
ISU84_RS13200	2768557..2769768	+	1212	WP_071788120.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T180137 WP_000813794.1 NZ_CP064103:c2765390-2765214 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T180137 NZ_CP085070:c133656-133351 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT180137 WP_001270286.1 NZ_CP064103:c2765168-2764752 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT180137 NZ_CP085070:c133876-133658 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• PDB: 6HPB