Detailed information of TA system TA180122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 1327438..1328074
• Replicon: chromosome
• Accession: NZ_CP064097
• Organism: Priestia aryabhattai strain B2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: IRA68_RS06960
• Protein ID: WP_013055004.1
• Coordinates: 1327724..1328074 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: IRA68_RS06955
• Protein ID: WP_013055003.1
• Coordinates: 1327438..1327719 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRA68_RS06930	1322804..1323775	+	972	WP_033580801.1	UV DNA damage repair endonuclease UvsE	-
IRA68_RS06935	1323784..1324386	-	603	WP_243495303.1	rhomboid family intramembrane serine protease	-
IRA68_RS06940	1324451..1324816	+	366	WP_028411881.1	holo-ACP synthase	-
IRA68_RS06945	1324875..1325933	+	1059	WP_033580802.1	outer membrane lipoprotein carrier protein LolA	-
IRA68_RS06950	1326047..1327237	+	1191	WP_243495304.1	alanine racemase	-
IRA68_RS06955	1327438..1327719	+	282	WP_013055003.1	hypothetical protein	Antitoxin
IRA68_RS06960	1327724..1328074	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
IRA68_RS06965	1328232..1329068	+	837	WP_013055005.1	RsbT co-antagonist protein RsbRA	-
IRA68_RS06970	1329071..1329427	+	357	WP_013055006.1	STAS domain-containing protein	-
IRA68_RS06975	1329431..1329832	+	402	WP_013055007.1	anti-sigma regulatory factor	-
IRA68_RS06980	1329846..1330856	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
IRA68_RS06985	1330916..1331248	+	333	WP_013055009.1	anti-sigma factor antagonist	-
IRA68_RS06990	1331245..1331730	+	486	WP_025753516.1	anti-sigma B factor RsbW	-
IRA68_RS06995	1331696..1332490	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T180122 WP_013055004.1 NZ_CP064097:1327724-1328074 [Priestia aryabhattai]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T180122 NZ_CP085069:1850943-1851197 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT180122 WP_013055003.1 NZ_CP064097:1327438-1327719 [Priestia aryabhattai]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT180122 NZ_CP085069:1850695-1850946 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9