Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 3120033..3120669 | Replicon | chromosome |
| Accession | NZ_CP064096 | ||
| Organism | Bacillus subtilis strain N1142-3at | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | IRJ28_RS16480 | Protein ID | WP_003156187.1 |
| Coordinates | 3120033..3120383 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G4NU32 |
| Locus tag | IRJ28_RS16485 | Protein ID | WP_003225183.1 |
| Coordinates | 3120388..3120669 (-) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IRJ28_RS16440 (3115077) | 3115077..3115676 | - | 600 | WP_003246608.1 | phosphoserine phosphatase RsbX | - |
| IRJ28_RS16445 (3115676) | 3115676..3116464 | - | 789 | WP_003246715.1 | RNA polymerase sigma factor SigB | - |
| IRJ28_RS16450 (3116430) | 3116430..3116912 | - | 483 | WP_003234299.1 | anti-sigma B factor RsbW | - |
| IRJ28_RS16455 (3116909) | 3116909..3117238 | - | 330 | WP_003234298.1 | anti-sigma factor antagonist RsbV | - |
| IRJ28_RS16460 (3117300) | 3117300..3118307 | - | 1008 | WP_003234295.1 | phosphoserine phosphatase RsbU | - |
| IRJ28_RS16465 (3118319) | 3118319..3118720 | - | 402 | WP_003246640.1 | serine/threonine-protein kinase RsbT | - |
| IRJ28_RS16470 (3118724) | 3118724..3119089 | - | 366 | WP_003225190.1 | RsbT antagonist protein RsbS | - |
| IRJ28_RS16475 (3119094) | 3119094..3119918 | - | 825 | WP_009966610.1 | RsbT co-antagonist protein RsbRA | - |
| IRJ28_RS16480 (3120033) | 3120033..3120383 | - | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| IRJ28_RS16485 (3120388) | 3120388..3120669 | - | 282 | WP_003225183.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| IRJ28_RS16490 (3120785) | 3120785..3121954 | - | 1170 | WP_003234284.1 | alanine racemase | - |
| IRJ28_RS16495 (3122069) | 3122069..3123085 | - | 1017 | WP_003234282.1 | outer membrane lipoprotein carrier protein LolA | - |
| IRJ28_RS16500 (3123251) | 3123251..3123616 | - | 366 | WP_003234281.1 | holo-ACP synthase | - |
| IRJ28_RS16505 (3123711) | 3123711..3124310 | + | 600 | WP_003246687.1 | rhomboid family intramembrane serine protease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T180121 WP_003156187.1 NZ_CP064096:c3120383-3120033 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T180121 NZ_CP085069:1850695-1850946 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10546.92 Da Isoelectric Point: 6.4708
>AT180121 WP_003225183.1 NZ_CP064096:c3120669-3120388 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT180121 NZ_CP085069:1850943-1851197 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|