Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 1440089..1440725 | Replicon | chromosome |
| Accession | NZ_CP064095 | ||
| Organism | Bacillus subtilis strain N1303-2Ay | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | IRJ27_RS07310 | Protein ID | WP_003156187.1 |
| Coordinates | 1440089..1440439 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G4NU32 |
| Locus tag | IRJ27_RS07315 | Protein ID | WP_003225183.1 |
| Coordinates | 1440444..1440725 (-) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IRJ27_RS07270 (1435133) | 1435133..1435732 | - | 600 | WP_003246608.1 | phosphoserine phosphatase RsbX | - |
| IRJ27_RS07275 (1435732) | 1435732..1436520 | - | 789 | WP_003246715.1 | RNA polymerase sigma factor SigB | - |
| IRJ27_RS07280 (1436486) | 1436486..1436968 | - | 483 | WP_003234299.1 | anti-sigma B factor RsbW | - |
| IRJ27_RS07285 (1436965) | 1436965..1437294 | - | 330 | WP_003234298.1 | anti-sigma factor antagonist RsbV | - |
| IRJ27_RS07290 (1437356) | 1437356..1438363 | - | 1008 | WP_003234295.1 | phosphoserine phosphatase RsbU | - |
| IRJ27_RS07295 (1438375) | 1438375..1438776 | - | 402 | WP_003246640.1 | serine/threonine-protein kinase RsbT | - |
| IRJ27_RS07300 (1438780) | 1438780..1439145 | - | 366 | WP_003225190.1 | RsbT antagonist protein RsbS | - |
| IRJ27_RS07305 (1439150) | 1439150..1439974 | - | 825 | WP_009966610.1 | RsbT co-antagonist protein RsbRA | - |
| IRJ27_RS07310 (1440089) | 1440089..1440439 | - | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| IRJ27_RS07315 (1440444) | 1440444..1440725 | - | 282 | WP_003225183.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| IRJ27_RS07320 (1440841) | 1440841..1442010 | - | 1170 | WP_003234284.1 | alanine racemase | - |
| IRJ27_RS07325 (1442125) | 1442125..1443141 | - | 1017 | WP_003234282.1 | outer membrane lipoprotein carrier protein LolA | - |
| IRJ27_RS07330 (1443307) | 1443307..1443672 | - | 366 | WP_003234281.1 | holo-ACP synthase | - |
| IRJ27_RS07335 (1443767) | 1443767..1444366 | + | 600 | WP_003246687.1 | rhomboid family intramembrane serine protease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T180077 WP_003156187.1 NZ_CP064095:c1440439-1440089 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T180077 NZ_CP085061:c5470-5195 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 94 a.a. Molecular weight: 10546.92 Da Isoelectric Point: 6.4708
>AT180077 WP_003225183.1 NZ_CP064095:c1440725-1440444 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT180077 NZ_CP085061:c5754-5470 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|