Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 610029..610945 Replicon chromosome
Accession NZ_CP064095
Organism Bacillus subtilis strain N1303-2Ay

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag IRJ27_RS02855 Protein ID WP_003244695.1
Coordinates 610029..610775 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag IRJ27_RS02860 Protein ID WP_003232646.1
Coordinates 610775..610945 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRJ27_RS02830 (605110) 605110..605256 - 147 WP_003244977.1 hypothetical protein -
IRJ27_RS02835 (605357) 605357..606307 - 951 WP_003245086.1 ring-cleaving dioxygenase -
IRJ27_RS02840 (606696) 606696..608012 + 1317 WP_003244819.1 serine/threonine exchanger -
IRJ27_RS02845 (608288) 608288..608905 + 618 WP_003218470.1 DUF47 domain-containing protein -
IRJ27_RS02850 (608918) 608918..609919 + 1002 WP_003232642.1 inorganic phosphate transporter -
IRJ27_RS02855 (610029) 610029..610775 + 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
IRJ27_RS02860 (610775) 610775..610945 + 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
IRJ27_RS02865 (611031) 611031..611168 + 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
IRJ27_RS02870 (611205) 611205..612098 - 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
IRJ27_RS02875 (612111) 612111..612374 - 264 WP_003232653.1 phage holin -
IRJ27_RS02880 (612387) 612387..612656 - 270 WP_003232655.1 hemolysin XhlA family protein -
IRJ27_RS02885 (612709) 612709..613548 - 840 WP_003245597.1 phage-like element PBSX protein XepA -
IRJ27_RS02890 (613592) 613592..613756 - 165 WP_003232658.1 XkdX family protein -
IRJ27_RS02895 (613753) 613753..614082 - 330 WP_003232660.1 XkdW family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T180076 WP_003244695.1 NZ_CP064095:610029-610775 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T180076 NZ_CP085060:c4452348-4452241 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT180076 WP_003232646.1 NZ_CP064095:610775-610945 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT180076 NZ_CP085060:4452396-4452462 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References