Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 951249..951885 Replicon chromosome
Accession NZ_CP064094
Organism Bacillus subtilis strain N1108-5at

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag IRJ26_RS05085 Protein ID WP_003156187.1
Coordinates 951249..951599 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag IRJ26_RS05090 Protein ID WP_003225183.1
Coordinates 951604..951885 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRJ26_RS05045 (946293) 946293..946892 - 600 WP_003246608.1 phosphoserine phosphatase RsbX -
IRJ26_RS05050 (946892) 946892..947680 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
IRJ26_RS05055 (947646) 947646..948128 - 483 WP_003234299.1 anti-sigma B factor RsbW -
IRJ26_RS05060 (948125) 948125..948454 - 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
IRJ26_RS05065 (948516) 948516..949523 - 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
IRJ26_RS05070 (949535) 949535..949936 - 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
IRJ26_RS05075 (949940) 949940..950305 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
IRJ26_RS05080 (950310) 950310..951134 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
IRJ26_RS05085 (951249) 951249..951599 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IRJ26_RS05090 (951604) 951604..951885 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
IRJ26_RS05095 (952001) 952001..953170 - 1170 WP_003234284.1 alanine racemase -
IRJ26_RS05100 (953285) 953285..954301 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
IRJ26_RS05105 (954467) 954467..954832 - 366 WP_003234281.1 holo-ACP synthase -
IRJ26_RS05110 (954927) 954927..955526 + 600 WP_003246687.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T180054 WP_003156187.1 NZ_CP064094:c951599-951249 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T180054 NZ_CP085060:291031-291204 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT180054 WP_003225183.1 NZ_CP064094:c951885-951604 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT180054 NZ_CP085060:290707-291003 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References