Detailed information of TA system TA180053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 121194..122110
• Replicon: chromosome
• Accession: NZ_CP064094
• Organism: Bacillus subtilis strain N1108-5at

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: IRJ26_RS00630
• Protein ID: WP_003244695.1
• Coordinates: 121194..121940 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: IRJ26_RS00635
• Protein ID: WP_003232646.1
• Coordinates: 121940..122110 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRJ26_RS00605 (116275)	116275..116421	-	147	WP_003244977.1	hypothetical protein	-
IRJ26_RS00610 (116522)	116522..117472	-	951	WP_003245086.1	ring-cleaving dioxygenase	-
IRJ26_RS00615 (117861)	117861..119177	+	1317	WP_003244819.1	serine/threonine exchanger	-
IRJ26_RS00620 (119453)	119453..120070	+	618	WP_003218470.1	DUF47 domain-containing protein	-
IRJ26_RS00625 (120083)	120083..121084	+	1002	WP_003232642.1	inorganic phosphate transporter	-
IRJ26_RS00630 (121194)	121194..121940	+	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
IRJ26_RS00635 (121940)	121940..122110	+	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
IRJ26_RS00640 (122196)	122196..122333	+	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
IRJ26_RS00645 (122370)	122370..123263	-	894	WP_003245230.1	N-acetylmuramoyl-L-alanine amidase	-
IRJ26_RS00650 (123276)	123276..123539	-	264	WP_003232653.1	phage holin	-
IRJ26_RS00655 (123552)	123552..123821	-	270	WP_003232655.1	hemolysin XhlA family protein	-
IRJ26_RS00660 (123874)	123874..124713	-	840	WP_003245597.1	phage-like element PBSX protein XepA	-
IRJ26_RS00665 (124757)	124757..124921	-	165	WP_003232658.1	XkdX family protein	-
IRJ26_RS00670 (124918)	124918..125247	-	330	WP_003232660.1	XkdW family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T180053 WP_003244695.1 NZ_CP064094:121194-121940 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T180053 NZ_CP085058:c61290-60910 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCAGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT180053 WP_003232646.1 NZ_CP064094:121940-122110 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT180053 NZ_CP085058:c61511-61290 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• AlphaFold DB: G4EXD8