180030

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 534980..535616 Replicon chromosome
Accession NZ_CP064093
Organism Bacillus subtilis strain N1282-4at

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag IRJ24_RS02825 Protein ID WP_003156187.1
Coordinates 534980..535330 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag IRJ24_RS02830 Protein ID WP_003225183.1
Coordinates 535335..535616 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRJ24_RS02785 (530024) 530024..530623 - 600 WP_003246608.1 phosphoserine phosphatase RsbX -
IRJ24_RS02790 (530623) 530623..531411 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
IRJ24_RS02795 (531377) 531377..531859 - 483 WP_003234299.1 anti-sigma B factor RsbW -
IRJ24_RS02800 (531856) 531856..532185 - 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
IRJ24_RS02805 (532247) 532247..533254 - 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
IRJ24_RS02810 (533266) 533266..533667 - 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
IRJ24_RS02815 (533671) 533671..534036 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
IRJ24_RS02820 (534041) 534041..534865 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
IRJ24_RS02825 (534980) 534980..535330 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IRJ24_RS02830 (535335) 535335..535616 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
IRJ24_RS02835 (535732) 535732..536901 - 1170 WP_003234284.1 alanine racemase -
IRJ24_RS02840 (537016) 537016..538032 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
IRJ24_RS02845 (538198) 538198..538563 - 366 WP_003234281.1 holo-ACP synthase -
IRJ24_RS02850 (538658) 538658..539257 + 600 WP_003246687.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T180030 WP_003156187.1 NZ_CP064093:c535330-534980 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T180030 NZ_CP085039:c376470-376279 [Streptomyces sp. WA1-19]
ATGACCTTCGTCGTGCTCTACGACGCGTGCGTGCTGTATCCGGACACACTGCGGGACCTGCTGGTCAGGGTCGCCCGGAC
AGGTCTGGTCCGCGCCCGGTGGACGGAGGCGATCCTGGACGAGGTGGACCGGGGCATCGCACGGAACCACCAGATCCCGC
CCGAGAAGCTGGCGCCCGCAGAACCCTCATGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT180030 WP_003225183.1 NZ_CP064093:c535616-535335 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT180030 NZ_CP085039:c376693-376472 [Streptomyces sp. WA1-19]
GTGGGGACGCACAGCGTGCAGCAGGCCGCCGACATGCTGAACGTCTCCCGCCCCTACCTGATCGGACTCCTGGAGTCCGG
GGAGATCCCCTTCAGGCTCGTGGGCCGCCACCGCCGCATCCGCTTCGCGGACCTGCGCCAGTACATCCGGGAGGACGACG
CGGCACGCAAGGGAGCGGCCGACGACCTGATGGAGCTGGACCAGGAGCTGGGACTGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References