Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3830468..3831104 Replicon chromosome
Accession NZ_CP064092
Organism Bacillus licheniformis strain C5

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag IRJ22_RS19720 Protein ID WP_003179128.1
Coordinates 3830754..3831104 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag IRJ22_RS19715 Protein ID WP_006638778.1
Coordinates 3830468..3830749 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRJ22_RS19695 3825583..3827064 + 1482 WP_009330132.1 PH domain-containing protein -
IRJ22_RS19700 3827061..3827660 - 600 WP_003179118.1 rhomboid family intramembrane serine protease -
IRJ22_RS19705 3828003..3828962 + 960 WP_152847888.1 outer membrane lipoprotein carrier protein LolA -
IRJ22_RS19710 3829187..3830356 + 1170 WP_025805944.1 alanine racemase -
IRJ22_RS19715 3830468..3830749 + 282 WP_006638778.1 hypothetical protein Antitoxin
IRJ22_RS19720 3830754..3831104 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IRJ22_RS19725 3831222..3832049 + 828 WP_003179130.1 RsbT co-antagonist protein RsbRA -
IRJ22_RS19730 3832053..3832418 + 366 WP_003179132.1 STAS domain-containing protein -
IRJ22_RS19735 3832421..3832822 + 402 WP_003179135.1 anti-sigma regulatory factor -
IRJ22_RS19740 3832833..3833840 + 1008 WP_003179137.1 PP2C family protein-serine/threonine phosphatase -
IRJ22_RS19745 3833899..3834225 + 327 WP_003179140.1 anti-sigma factor antagonist -
IRJ22_RS19750 3834225..3834710 + 486 WP_003179142.1 anti-sigma B factor RsbW -
IRJ22_RS19755 3834676..3835467 + 792 WP_003179144.1 RNA polymerase sigma factor SigB -
IRJ22_RS19760 3835464..3836063 + 600 WP_003179145.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T180029 WP_003179128.1 NZ_CP064092:3830754-3831104 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T180029 NZ_CP085037:514001-514351 [Bacillus pumilus]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTTAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT180029 WP_006638778.1 NZ_CP064092:3830468-3830749 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT180029 NZ_CP085037:513715-513996 [Bacillus pumilus]
TTGTCTGAATCCAGCGCAACAAATGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAGTTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References