Detailed information of TA system TA180028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 479212..480131
• Replicon: chromosome
• Accession: NZ_CP064092
• Organism: Bacillus licheniformis strain C5

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: IRJ22_RS02565
• Protein ID: WP_003180879.1
• Coordinates: 479385..480131 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: T5HT37
• Locus tag: IRJ22_RS02560
• Protein ID: WP_003180877.1
• Coordinates: 479212..479385 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRJ22_RS02535	474637..475734	+	1098	WP_003180867.1	mannonate dehydratase	-
IRJ22_RS02540	475710..476558	+	849	WP_003180869.1	SDR family oxidoreductase	-
IRJ22_RS02545	476585..477598	+	1014	WP_011197810.1	zinc-binding alcohol dehydrogenase family protein	-
IRJ22_RS02550	477651..478922	+	1272	WP_011197811.1	MFS transporter	-
IRJ22_RS02555	478985..479116	-	132	WP_003180875.1	hypothetical protein	-
IRJ22_RS02560	479212..479385	-	174	WP_003180877.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
IRJ22_RS02565	479385..480131	-	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
IRJ22_RS02570	480242..481240	-	999	WP_009328723.1	inorganic phosphate transporter	-
IRJ22_RS02575	481253..481870	-	618	WP_003180884.1	DUF47 domain-containing protein	-
IRJ22_RS02580	482202..483959	+	1758	WP_003180886.1	gamma-glutamyltransferase	-
IRJ22_RS02585	484088..484732	+	645	WP_009328721.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T180028 WP_003180879.1 NZ_CP064092:c480131-479385 [Bacillus licheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T180028 NZ_CP085032:3703648-3703902 [Proteus terrae subsp. cibarius]
ATGAAATTAATTTTTTCTGAGCAATCATGGAGTGATTATCTTTATTGGCAACAAATAGACAAGAAAATAATTAAACGCAT
TAACGAACTCATAAAAGATATTAAAAGAACACCGTTTAGCGGAATAGGTAAACCAGAGCCCTTGAAACATAATTTAGCAG
GTTTTTGGTCAAGACGGATCACTGATGAACATCGCCTTATCTACCGTATTACTGATTCCGCTATAGAAATTGCATCATGT
CGATACCACTATTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6945.04 Da
• Isoelectric Point: 10.9186

>AT180028 WP_003180877.1 NZ_CP064092:c479385-479212 [Bacillus licheniformis]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT180028 NZ_CP085032:3703400-3703651 [Proteus terrae subsp. cibarius]
ATGAAAACGATAAATTATACAGAAGCAAGACAAAACTTAACTTCAATTATGAATGAAACAATTGATGATAGAGTACCCAT
CATGATCACAAGGCAAAAGGGTATTCCATGTGTTCTTTTATCTCTTGATGAATACCAAGCACTAGAAGAAACTGCCTATC
TGTTACGATCTCCAGCGAATGCGCGCCATTTGCTCGATTCTATTGATGAACTGAACCAAGGTAAAGGTATAGAAAAGGAT
ATAACGGAATGA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: T5HT37