Detailed information of TA system TA180027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3866781..3867698
• Replicon: chromosome
• Accession: NZ_CP064091
• Organism: Bacillus velezensis strain C1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: IRJ21_RS19150
• Protein ID: WP_007407256.1
• Coordinates: 3866952..3867698 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: IRJ21_RS19145
• Protein ID: WP_003154807.1
• Coordinates: 3866781..3866951 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRJ21_RS19105	3861993..3863582	+	1590	WP_029973066.1	hypothetical protein	-
IRJ21_RS19110	3863595..3864020	+	426	WP_029973065.1	hypothetical protein	-
IRJ21_RS19115	3864025..3864222	+	198	WP_007610833.1	XkdX family protein	-
IRJ21_RS19120	3864279..3865040	+	762	WP_182072616.1	phage portal protein	-
IRJ21_RS19125	3865092..3865355	+	264	WP_021493821.1	hemolysin XhlA family protein	-
IRJ21_RS19130	3865369..3865632	+	264	WP_182072618.1	phage holin	-
IRJ21_RS19135	3865646..3866524	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
IRJ21_RS19140	3866559..3866684	-	126	WP_003154809.1	hypothetical protein	-
IRJ21_RS19145	3866781..3866951	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
IRJ21_RS19150	3866952..3867698	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
IRJ21_RS19155	3867802..3868800	-	999	WP_003154805.1	inorganic phosphate transporter	-
IRJ21_RS19160	3868813..3869430	-	618	WP_003154804.1	DUF47 domain-containing protein	-
IRJ21_RS19165	3869716..3871032	-	1317	WP_061862107.1	amino acid permease	-
IRJ21_RS19170	3871355..3872305	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3819963..3904517	84554

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T180027 WP_007407256.1 NZ_CP064091:c3867698-3866952 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T180027 NZ_CP085032:3703400-3703651 [Proteus terrae subsp. cibarius]
ATGAAAACGATAAATTATACAGAAGCAAGACAAAACTTAACTTCAATTATGAATGAAACAATTGATGATAGAGTACCCAT
CATGATCACAAGGCAAAAGGGTATTCCATGTGTTCTTTTATCTCTTGATGAATACCAAGCACTAGAAGAAACTGCCTATC
TGTTACGATCTCCAGCGAATGCGCGCCATTTGCTCGATTCTATTGATGAACTGAACCAAGGTAAAGGTATAGAAAAGGAT
ATAACGGAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT180027 WP_003154807.1 NZ_CP064091:c3866951-3866781 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT180027 NZ_CP085032:3703648-3703902 [Proteus terrae subsp. cibarius]
ATGAAATTAATTTTTTCTGAGCAATCATGGAGTGATTATCTTTATTGGCAACAAATAGACAAGAAAATAATTAAACGCAT
TAACGAACTCATAAAAGATATTAAAAGAACACCGTTTAGCGGAATAGGTAAACCAGAGCCCTTGAAACATAATTTAGCAG
GTTTTTGGTCAAGACGGATCACTGATGAACATCGCCTTATCTACCGTATTACTGATTCCGCTATAGAAATTGCATCATGT
CGATACCACTATTAG

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14