Detailed information of TA system TA180026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3041122..3041759
• Replicon: chromosome
• Accession: NZ_CP064091
• Organism: Bacillus velezensis strain C1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: IRJ21_RS14715
• Protein ID: WP_003156187.1
• Coordinates: 3041409..3041759 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: IRJ21_RS14710
• Protein ID: WP_003156188.1
• Coordinates: 3041122..3041403 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRJ21_RS14690	3037487..3038086	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
IRJ21_RS14695	3038179..3038544	+	366	WP_014304402.1	holo-ACP synthase	-
IRJ21_RS14700	3038709..3039716	+	1008	WP_182072313.1	outer membrane lipoprotein carrier protein LolA	-
IRJ21_RS14705	3039833..3041002	+	1170	WP_182072314.1	alanine racemase	-
IRJ21_RS14710	3041122..3041403	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
IRJ21_RS14715	3041409..3041759	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
IRJ21_RS14720	3041877..3042698	+	822	WP_003156182.1	STAS domain-containing protein	-
IRJ21_RS14725	3042703..3043068	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
IRJ21_RS14730	3043071..3043472	+	402	WP_003156178.1	anti-sigma regulatory factor	-
IRJ21_RS14735	3043484..3044491	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
IRJ21_RS14740	3044555..3044884	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
IRJ21_RS14745	3044881..3045363	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
IRJ21_RS14750	3045329..3046117	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
IRJ21_RS14755	3046117..3046719	+	603	WP_007410234.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T180026 WP_003156187.1 NZ_CP064091:3041409-3041759 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T180026 NZ_CP085032:3661501-3661887 [Proteus terrae subsp. cibarius]
ATGGTTATCTATAAAACAAAGATGTTCAAAAACAGTTTCAAGAAAATGCCTATCAATGATGCTGCTTTAATAGATATTGC
CAACGAAGTATATGCAGGACACTTTGAAGCTGACTTAGGTGGGGGGATTATCAAAAAACGGATCTGTATCCATGGAAAAG
GAAAGAGTAGTGGAATTAGAACAATTATATTTTATAAACAGGGAAGTAATTTATTCTTTGCTGATGGATGGAAAAAATCT
CACTTGAGCGCTAAAAAATCTAAAGAAATTACAGATGATGAACTAGAATCCTATAAAGATCTGGCAAAAGATCTCTTCAG
TGCAACTAAAAATAAAATAGATAAGATGGTTGCACTAGGCCTTTTAACGGAGGTTAAACATGACTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT180026 WP_003156188.1 NZ_CP064091:3041122-3041403 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT180026 NZ_CP085032:3661880-3662200 [Proteus terrae subsp. cibarius]
ATGACTGATAAAATGCAAGAAATGCTGAAATTTGCACAAGAAATGGAAGTTGCGGGAATAACCGATGGAGAATCGGCTAG
ACGAATTGCAGCGAGAATAAAAGCCAAAGAATTAAAAGAAAGATATGCTCACCTGACTCCAATGAATGGTGAAGATATAA
AAAGAATTCGCGAGCAAAATAATATTTCGCAATCGTCTCTTGCTTTAATACTAAATATGACAAAAGAGAGCGTATCAAAA
TGGGAGCGGAACGAAATTAAACCCAATGGAGCTGCATTGAGACTTTTGAATTTAATAGATAATAAAGCAATTTCAATCTA
A

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5