180025

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 354303..354956 Replicon chromosome
Accession NZ_CP064090
Organism Brevibacillus parabrevis strain B3

Toxin (Protein)


Gene name mazF Uniprot ID J2PLP3
Locus tag IRJ17_RS01600 Protein ID WP_005830205.1
Coordinates 354303..354653 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3M8AYL7
Locus tag IRJ17_RS01605 Protein ID WP_025845698.1
Coordinates 354657..354956 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRJ17_RS01575 351034..351831 - 798 WP_122966574.1 RNA polymerase sigma factor SigB -
IRJ17_RS01580 351800..352294 - 495 WP_122966575.1 anti-sigma B factor RsbW -
IRJ17_RS01585 352294..352620 - 327 WP_063229461.1 anti-sigma factor antagonist -
IRJ17_RS01590 352646..353647 - 1002 WP_122966576.1 PP2C family protein-serine/threonine phosphatase -
IRJ17_RS01595 353641..353997 - 357 WP_229049930.1 anti-sigma regulatory factor -
IRJ17_RS01600 354303..354653 - 351 WP_005830205.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IRJ17_RS01605 354657..354956 - 300 WP_025845698.1 CopG family ribbon-helix-helix protein Antitoxin
IRJ17_RS01610 355135..356337 - 1203 WP_242772907.1 alanine racemase -
IRJ17_RS01615 356340..356780 - 441 WP_122966579.1 hypothetical protein -
IRJ17_RS01620 357002..358138 - 1137 WP_242772909.1 hypothetical protein -
IRJ17_RS01625 358337..359374 - 1038 WP_122966581.1 outer membrane lipoprotein-sorting protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-52)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12917.86 Da        Isoelectric Point: 4.8435

>T180025 WP_005830205.1 NZ_CP064090:c354653-354303 [Brevibacillus parabrevis]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Download         Length: 351 bp

>T180025 NZ_CP085032:3365392-3365865 [Proteus terrae subsp. cibarius]
ATGGATTTCCCTACAGATTTGAATGGATGGACAATCTACGCACATCCTTGCTTTAAACAACAATATGATGAATTACTACA
AAAAGTCGAAGCTCTGAAAAAAAAATATCCCGAAGATTATCAGAAAAAAGCAGATACAAAAATTTTTGCTCATGTATTGA
AATCTATAGTAAACATCACAAATGACCCACGGGCACCAGAGTTTAGGTTGGGTAATACTTTAGGAGAAGAAAATAAAAAT
TGGTCACGAATTAAATTAGTAAATGGAAGATATCGTTTATTTTTTAGATTCAGTTTTAAGGAAAAGATCATCATTCTGGC
ATGGATAAATGATAATGATACTTTGCGAACTTATGGCAATAAAACTGATGCTTACCGAGTCTTTGAGAAAAAACTAAAGA
AAGGTCATCCACCTAAAAATTGGAATGATTTATTTAGTGACTGTATAAATGAAGCTGACTGTCAGGATCCTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11323.26 Da        Isoelectric Point: 10.3594

>AT180025 WP_025845698.1 NZ_CP064090:c354956-354657 [Brevibacillus parabrevis]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLKMASE
AFGAEEEADHTLVRLVSGV

Download         Length: 300 bp

>AT180025 NZ_CP085032:3365050-3365385 [Proteus terrae subsp. cibarius]
ATGCTAACCCACGTTTGCACTGACAACATCGCTCTTGAAGCTGATTCAAAAATAACAGAACGTCATCAAACCACAATTCC
CGCGTCAATTAGAGAAGCTCTACATCTTAGTGGTGGAGATCGTATTCACTACAAATTACTAAGCACTGGTGATGTACTTA
TCTCACGTCACAGCGATGGATCTGAAGATCCTGTTATGGCATCGTTTCTTAATTTTCTCGCTCAAGATATAACTAATCAT
CCTCAAACCATTCAACCTCTCGATATATCTCGTGGTTTTGAATTAGTTGAAGGTATGGATATCAACCTTGACGATGACAT
CACCGATAACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J2PLP3

Antitoxin

Source ID Structure
AlphaFold DB A0A3M8AYL7

References