Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 858822..859458 Replicon chromosome
Accession NZ_CP064089
Organism Bacillus altitudinis strain N435-3

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag IRB21_RS04170 Protein ID WP_003214169.1
Coordinates 859108..859458 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag IRB21_RS04165 Protein ID WP_003214273.1
Coordinates 858822..859103 (+) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRB21_RS04145 854976..855581 - 606 WP_007496491.1 rhomboid family intramembrane serine protease -
IRB21_RS04150 855676..856041 + 366 WP_019744212.1 holo-ACP synthase -
IRB21_RS04155 856202..857218 + 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
IRB21_RS04160 857347..858528 + 1182 WP_242731620.1 alanine racemase -
IRB21_RS04165 858822..859103 + 282 WP_003214273.1 hypothetical protein Antitoxin
IRB21_RS04170 859108..859458 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IRB21_RS04175 859573..860403 + 831 WP_058336383.1 RsbT co-antagonist protein RsbRA -
IRB21_RS04180 860408..860776 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
IRB21_RS04185 860779..861180 + 402 WP_003214085.1 anti-sigma regulatory factor -
IRB21_RS04190 861191..862198 + 1008 WP_017358394.1 PP2C family protein-serine/threonine phosphatase -
IRB21_RS04195 862258..862587 + 330 WP_017358393.1 anti-sigma factor antagonist -
IRB21_RS04200 862584..863072 + 489 WP_048001251.1 anti-sigma B factor RsbW -
IRB21_RS04205 863038..863826 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
IRB21_RS04210 863826..864425 + 600 WP_024719202.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-113)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T180024 WP_003214169.1 NZ_CP064089:859108-859458 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T180024 NZ_CP085032:2355967-2356458 [Proteus terrae subsp. cibarius]
ATGGGAAAAGTAACAGCACCTGAGCCGTTATCAAACTCACATGAAGTGGCTGATTTTTATAGTAGTGAAAATGTATTAGA
TAACTGGATAAAACAAAGAGGTTTAAAAAACCAATTTTTAGGTGCATCCCGTACATTTGTGGTCTGTGAAAAAAATAAAA
AACGTGTTGTTGGGTACTATTCTTTAGCAACAGGTAGTGTTAACCATACTGAAGCAATGAGTCATATTCGTCGCAATATG
CCAGACCCAATACCCGTGATCATTTTAGCTAGACTTGCTGTTGATAAAACTTTTCATGGAAAAGGTCTTGGTGCCGATTT
GTTAAGAGATGCAGTTTTGCGTTGTCATCATGTAGCTGAAAACATTGGTGTTAGAGCGATTATGGTTCATGCTTTAACTG
AAAACGCAAAACATTTCTACCTTCATCATGGTTTCAAAGCTTCTTCGATTCAAGACAAAATCTTATTCTTAGCACTAAAA
AAGAAAATATAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT180024 WP_003214273.1 NZ_CP064089:858822-859103 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT180024 NZ_CP085032:2355713-2355979 [Proteus terrae subsp. cibarius]
ATGAAAGCTGATGTACAACTCAATATTCGCGCTAAAGAATCACAACGCGCACTTATTGATACTGCTGCTGAAATTCTCCA
TAAATCTCGTACTGACTTTATTCTTGAGACAGCATGCCAAGCTGCTGAAGACGTCATTTTAGACCGTAGAACCTTTAATT
TAAATGATGCTCAATATGCGGAATTTATAGAAATTATTGATGCGCCGGTTGATATTGACCCTGCTCTTGAAAAGTTATTA
ATGAGAAAACCATTATGGGAAAAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB K2MHT4


Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References