Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1898560..1899191 Replicon chromosome
Accession NZ_CP064062
Organism Brucella anthropi strain PBO

Toxin (Protein)


Gene name vapC Uniprot ID A0A256GHB5
Locus tag IR196_RS08840 Protein ID WP_029928525.1
Coordinates 1898560..1898961 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A256GGW7
Locus tag IR196_RS08845 Protein ID WP_010660354.1
Coordinates 1898961..1899191 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IR196_RS08820 1893997..1895517 - 1521 WP_029928517.1 FAD-dependent oxidoreductase -
IR196_RS08825 1895647..1896552 - 906 WP_094515270.1 LysR family transcriptional regulator -
IR196_RS08830 1896713..1897873 - 1161 WP_029928521.1 mandelate racemase/muconate lactonizing enzyme family protein -
IR196_RS08835 1897902..1898156 - 255 WP_029928523.1 hypothetical protein -
IR196_RS08840 1898560..1898961 - 402 WP_029928525.1 tRNA(fMet)-specific endonuclease VapC Toxin
IR196_RS08845 1898961..1899191 - 231 WP_010660354.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IR196_RS08850 1899776..1900720 - 945 WP_194531748.1 helix-turn-helix domain-containing protein -
IR196_RS08855 1900710..1901402 - 693 WP_151895871.1 HAD family hydrolase -
IR196_RS08860 1901671..1902933 + 1263 WP_192931042.1 extracellular solute-binding protein -
IR196_RS08865 1902938..1903888 + 951 WP_151895738.1 ABC transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1770523..2015114 244591


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-124)

Antitoxin

SpoVT-AbrB domain

(7-54)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 15055.14 Da        Isoelectric Point: 6.8629

>T180021 WP_029928525.1 NZ_CP064062:c1898961-1898560 [Brucella anthropi]
MLKYMLDTNICIFTIKNRPQQVREAFNRFHDQLCISSVSLMELIYGAEKSAHPEKNLAVVEGFAARLEVLSYDEPAANHT
GQLRAELARSGTPIGPYDQLIAGHARSRGLIIVTNNRREFDRVPGLRVEDWTD

Download         Length: 402 bp

>T180021 NZ_CP084987:c130755-130471 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTAGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8487.53 Da        Isoelectric Point: 4.5045

>AT180021 WP_010660354.1 NZ_CP064062:c1899191-1898961 [Brucella anthropi]
MEQGAVFQSNRSQAIRLPKAVALPADVKRVDIVAVGRTRIIAPAGEVWDSWFDSLEATSDFMNERDQPAPQEREAF

Download         Length: 231 bp

>AT180021 NZ_CP084987:c130993-130745 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGTACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTCAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCTGTAATCGAGGTCAGTCTAGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A256GHB5

Antitoxin

Source ID Structure
AlphaFold DB A0A256GGW7

References