Detailed information of TA system TA180020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1869606..1870180
• Replicon: chromosome
• Accession: NZ_CP064062
• Organism: Brucella anthropi strain PBO

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IR196_RS08705
• Protein ID: WP_010660356.1
• Coordinates: 1869803..1870180 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A849KU45
• Locus tag: IR196_RS08700
• Protein ID: WP_010660355.1
• Coordinates: 1869606..1869806 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IR196_RS08685	1865226..1865819	-	594	WP_010660339.1	biliverdin-producing heme oxygenase	-
IR196_RS08690	1865870..1866598	-	729	WP_194531747.1	Crp/Fnr family transcriptional regulator	-
IR196_RS08695	1866861..1869395	+	2535	WP_010660341.1	GAF domain-containing protein	-
IR196_RS08700	1869606..1869806	+	201	WP_010660355.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IR196_RS08705	1869803..1870180	+	378	WP_010660356.1	type II toxin-antitoxin system VapC family toxin	Toxin
IR196_RS08710	1870371..1871261	-	891	WP_151612519.1	DMT family transporter	-
IR196_RS08715	1871370..1872530	-	1161	WP_029928473.1	PLP-dependent aminotransferase family protein	-
IR196_RS08720	1872593..1873957	-	1365	WP_151612780.1	MmgE/PrpD family protein	-
IR196_RS08725	1873968..1874852	-	885	WP_151612517.1	polysaccharide deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1770523..2015114	244591

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-110)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13961.17 Da
• Isoelectric Point: 7.0615

>T180020 WP_010660356.1 NZ_CP064062:1869803-1870180 [Brucella anthropi]
VILADTSIWIDHFRRGDNDLVKIIGNDLLLCHPAVIGELALGSLRDRTAVLTFLRAQRETIVATHDEVMTLIERHSVFSM
GIGYTDAHLLASVLLDQRTSLWTRDKRLKLAALKAGAALYEPFHS

Nucleotide

• Length: 378 bp

>T180020 NZ_CP084986:c5252852-5252469 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7162.26 Da
• Isoelectric Point: 11.1107

>AT180020 WP_010660355.1 NZ_CP064062:1869606-1869806 [Brucella anthropi]
MRSTINLDDNLLEKARSLTGTKETAALVRQALEALVRVETGKRLIALGGTMPDAEAAPRRRSSVNK

Nucleotide

• Length: 201 bp

>AT180020 NZ_CP084986:c5253094-5252852 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A849KU45