Detailed information of TA system TA180019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 3400949..3401585
• Replicon: chromosome
• Accession: NZ_CP064060
• Organism: Anoxybacillus caldiproteolyticus strain U458

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: L7ZVP1
• Locus tag: ISX45_RS17100
• Protein ID: WP_003253417.1
• Coordinates: 3401235..3401585 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: ISX45_RS17095
• Protein ID: WP_181557067.1
• Coordinates: 3400949..3401230 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISX45_RS17075	3397262..3397864	-	603	WP_181557064.1	rhomboid family intramembrane serine protease	-
ISX45_RS17080	3397934..3398296	+	363	WP_194520738.1	holo-ACP synthase	-
ISX45_RS17085	3398390..3399403	+	1014	WP_181557065.1	outer membrane lipoprotein carrier protein LolA	-
ISX45_RS17090	3399658..3400827	+	1170	WP_194519486.1	alanine racemase	-
ISX45_RS17095	3400949..3401230	+	282	WP_181557067.1	antitoxin endoai	Antitoxin
ISX45_RS17100	3401235..3401585	+	351	WP_003253417.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
ISX45_RS17105	3401740..3403911	+	2172	WP_194519487.1	RNA-binding transcriptional accessory protein	-
ISX45_RS17110	3403927..3404040	-	114	WP_012748966.1	cortex morphogenetic protein CmpA	-
ISX45_RS17115	3404124..3404579	+	456	WP_181557070.1	SprT family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12992.00 Da
• Isoelectric Point: 4.8781

>T180019 WP_003253417.1 NZ_CP064060:3401235-3401585 [Anoxybacillus caldiproteolyticus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T180019 NZ_CP084986:c4929334-4928417 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGTGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10659.21 Da
• Isoelectric Point: 7.0147

>AT180019 WP_181557067.1 NZ_CP064060:3400949-3401230 [Anoxybacillus caldiproteolyticus]
VSESSATTEIVVRLPQALLTELDVLVKQENGNRNELIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFLAEY
EADHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT180019 NZ_CP084986:c4930098-4929340 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A7U9ERQ4