Detailed information of TA system TA180010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 36346..37483
• Replicon: plasmid unnamed3
• Accession: NZ_CP064052
• Organism: Enterococcus faecium strain VRE3370

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: Q54944
• Locus tag: ISU52_RS15835
• Protein ID: WP_001284311.1
• Coordinates: 36346..37209 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: ISU52_RS15840
• Protein ID: WP_000301765.1
• Coordinates: 37211..37483 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU52_RS15805	31918..33213	-	1296	WP_002297218.1	ISL3-like element ISEfa11 family transposase	-
ISU52_RS15810	33458..33709	+	252	Protein_43	GNAT family N-acetyltransferase	-
ISU52_RS15815	33802..34596	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
ISU52_RS15820	35006..35089	+	84	Protein_45	peptide-binding protein	-
ISU52_RS15825	35138..35221	+	84	WP_031929417.1	23S rRNA methyltransferase attenuator leader peptide ErmL	-
ISU52_RS15830	35346..36083	+	738	WP_001038796.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
ISU52_RS15835	36346..37209	-	864	WP_001284311.1	toxin zeta	Toxin
ISU52_RS15840	37211..37483	-	273	WP_000301765.1	antitoxin	Antitoxin
ISU52_RS15845	37500..37709	-	210	WP_000527318.1	peptide-binding protein	-
ISU52_RS15850	37874..38554	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
ISU52_RS15855	38707..38931	+	225	WP_002363584.1	bacteriocin hiracin-JM79	-
ISU52_RS15860	38951..39238	+	288	WP_002363589.1	bacteriocin immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	lnu(B) / ant(6)-Ia / aph(3')-III / erm(B)	-	1..39679	39679
	-	inside	IScluster/Tn	lnu(B) / ant(6)-Ia / aph(3')-III / erm(B)	-	3658..38560	34902

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32404.02 Da
• Isoelectric Point: 6.9964

>T180010 WP_001284311.1 NZ_CP064052:c37209-36346 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T180010 NZ_CP084986:324618-324986 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT180010 WP_000301765.1 NZ_CP064052:c37483-37211 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT180010 NZ_CP084986:324400-324621 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 1GVN
• PDB: 3Q8X

Antitoxin

• PDB: 3Q8X
• PDB: 1GVN
• AlphaFold DB: A0A829F0A3