Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 27897..29005 Replicon plasmid unnamed3
Accession NZ_CP064052
Organism Enterococcus faecium strain VRE3370

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag ISU52_RS15780 Protein ID WP_002303392.1
Coordinates 28136..29005 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag ISU52_RS15775 Protein ID WP_002303393.1
Coordinates 27897..28121 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU52_RS15745 22948..23364 - 417 WP_001232859.1 hypothetical protein -
ISU52_RS15750 23367..23651 - 285 WP_139901088.1 hypothetical protein -
ISU52_RS15755 24351..24464 - 114 WP_194505306.1 type I toxin-antitoxin system Fst family toxin -
ISU52_RS15760 24506..25186 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
ISU52_RS15765 25442..26245 + 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
ISU52_RS15770 26933..27754 + 822 Protein_35 recombinase zinc beta ribbon domain-containing protein -
ISU52_RS15775 27897..28121 + 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
ISU52_RS15780 28136..29005 + 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
ISU52_RS15785 28986..29720 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
ISU52_RS15790 29753..30661 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
ISU52_RS15795 30658..30906 + 249 WP_002347175.1 hypothetical protein -
ISU52_RS15800 30909..31817 - 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
ISU52_RS15805 31918..33213 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
ISU52_RS15810 33458..33709 + 252 Protein_43 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 1..39679 39679
- inside IScluster/Tn lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 3658..38560 34902


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T180009 WP_002303392.1 NZ_CP064052:28136-29005 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T180009 NZ_CP084985:300008-300292 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTAACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT180009 WP_002303393.1 NZ_CP064052:27897-28121 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp

>AT180009 NZ_CP084985:299770-300018 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACCTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCATATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References