Detailed information of TA system TA180008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 48061..48668
• Replicon: plasmid unnamed2
• Accession: NZ_CP064051
• Organism: Enterococcus faecium strain VRE3370

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: ISU52_RS15490
• Protein ID: WP_002321032.1
• Coordinates: 48318..48668 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: ISU52_RS15485
• Protein ID: WP_002287514.1
• Coordinates: 48061..48324 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU52_RS15445	43097..43996	+	900	WP_002322787.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
ISU52_RS15450	43999..44484	+	486	WP_002296241.1	single-stranded DNA-binding protein	-
ISU52_RS15455	44850..45110	+	261	WP_002296240.1	hypothetical protein	-
ISU52_RS15460	45551..45757	+	207	WP_002296239.1	hypothetical protein	-
ISU52_RS15465	45757..46008	+	252	WP_002296238.1	hypothetical protein	-
ISU52_RS15470	46023..46460	+	438	WP_002296237.1	hypothetical protein	-
ISU52_RS15475	46453..47160	+	708	WP_002353391.1	hypothetical protein	-
ISU52_RS15480	47541..47720	+	180	WP_002305761.1	hypothetical protein	-
ISU52_RS15485	48061..48324	+	264	WP_002287514.1	hypothetical protein	Antitoxin
ISU52_RS15490	48318..48668	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ISU52_RS15495	48692..49306	-	615	WP_010729795.1	recombinase family protein	-
ISU52_RS15500	49620..50042	+	423	WP_002288787.1	hypothetical protein	-
ISU52_RS15505	50052..50255	+	204	WP_002299573.1	hypothetical protein	-
ISU52_RS15510	50466..51050	+	585	WP_002299575.1	recombinase family protein	-
ISU52_RS15515	51421..52746	+	1326	WP_002303115.1	Y-family DNA polymerase	-
ISU52_RS15520	52739..53089	+	351	WP_002303114.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..63187	63187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T180008 WP_002321032.1 NZ_CP064051:48318-48668 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T180008 NZ_CP084985:c195901-195458 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT180008 WP_002287514.1 NZ_CP064051:48061-48324 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT180008 NZ_CP084985:c196128-195898 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0