Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 16812..17331 Replicon plasmid unnamed1
Accession NZ_CP064050
Organism Enterococcus faecium strain VRE3370

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag ISU52_RS14110 Protein ID WP_000588503.1
Coordinates 17074..17331 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag ISU52_RS14105 Protein ID WP_000388479.1
Coordinates 16812..17081 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU52_RS14060 12009..12908 + 900 WP_002303312.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
ISU52_RS14065 12911..13396 + 486 WP_002303313.1 single-stranded DNA-binding protein -
ISU52_RS14070 13537..13740 - 204 WP_002316074.1 putative holin-like toxin -
ISU52_RS14075 13815..14075 + 261 WP_002295623.1 hypothetical protein -
ISU52_RS14080 14512..14718 + 207 WP_002347218.1 hypothetical protein -
ISU52_RS14085 14718..14969 + 252 WP_002296238.1 hypothetical protein -
ISU52_RS14090 14984..15421 + 438 WP_002300851.1 hypothetical protein -
ISU52_RS14095 15414..16121 + 708 WP_002303117.1 hypothetical protein -
ISU52_RS14100 16502..16681 + 180 WP_001120991.1 hypothetical protein -
ISU52_RS14105 16812..17081 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ISU52_RS14110 17074..17331 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
ISU52_RS14115 17790..17933 + 144 WP_164857621.1 hypothetical protein -
ISU52_RS14120 17968..18792 + 825 WP_001809248.1 hypothetical protein -
ISU52_RS14125 18957..19571 - 615 WP_001261742.1 recombinase family protein -
ISU52_RS14130 19809..20229 + 421 Protein_23 hypothetical protein -
ISU52_RS14135 20238..20484 + 247 Protein_24 hypothetical protein -
ISU52_RS14140 20647..21229 + 583 Protein_25 recombinase family protein -
ISU52_RS14145 21418..22098 - 681 WP_010861579.1 IS6-like element IS1216 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..219794 219794
- flank IS/Tn - - 21418..22098 680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T180006 WP_000588503.1 NZ_CP064050:17074-17331 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T180006 NZ_CP084984:c5290825-5290442 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT180006 WP_000388479.1 NZ_CP064050:16812-17081 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT180006 NZ_CP084984:c5291067-5290825 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References