Detailed information of TA system TA179989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 5654..6390
• Replicon: plasmid pBM404-3-1
• Accession: NZ_CP064045
• Organism: Klebsiella quasipneumoniae strain BM404-3-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: ISA72_RS25925
• Protein ID: WP_003026803.1
• Coordinates: 5908..6390 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: ISA72_RS25920
• Protein ID: WP_003026799.1
• Coordinates: 5654..5920 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA72_RS25875	1716..2078	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
ISA72_RS25880	2128..2478	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
ISA72_RS25885	2836..3105	+	270	WP_019706036.1	hypothetical protein	-
ISA72_RS25890	3093..3668	+	576	WP_004152103.1	hypothetical protein	-
ISA72_RS25895	3699..4193	+	495	WP_004152104.1	DNA-binding protein	-
ISA72_RS25900	4237..4605	+	369	WP_004152105.1	hypothetical protein	-
ISA72_RS25905	4639..4842	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
ISA72_RS25910	4891..5148	+	258	WP_004152107.1	hypothetical protein	-
ISA72_RS25915	5224..5478	+	255	WP_004152108.1	hypothetical protein	-
ISA72_RS25920	5654..5920	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
ISA72_RS25925	5908..6390	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
ISA72_RS25930	6591..7994	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
ISA72_RS25935	8023..8655	-	633	WP_001567369.1	hypothetical protein	-
ISA72_RS25940	8832..10086	-	1255	Protein_14	IS3 family transposase	-
ISA72_RS25945	10174..11136	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..163717	163717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T179989 WP_003026803.1 NZ_CP064045:5908-6390 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T179989 NZ_CP084924:c61417-61061 [Acinetobacter pittii]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAACCTACACAAGAGAAGGTCCTAGC
TGCCCTGGTTGTTCTACAGCAGCAGGGACCGAGTTTAGGCCGTCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGTGTTCAACATCGCGGTAGACCCTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACTACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179989 WP_003026799.1 NZ_CP064045:5654-5920 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179989 NZ_CP084924:c61068-60766 [Acinetobacter pittii]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCTGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGTGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAACGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66