Detailed information of TA system TA179954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1907115..1907785
• Replicon: chromosome
• Accession: NZ_CP064003
• Organism: Xanthomonas translucens pv. translucens strain XtKm9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ISN32_RS08295
• Protein ID: WP_058361064.1
• Coordinates: 1907115..1907534 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S7EM03
• Locus tag: ISN32_RS08300
• Protein ID: WP_003473422.1
• Coordinates: 1907531..1907785 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISN32_RS08250	1902150..1902383	+	234	WP_003472509.1	hypothetical protein	-
ISN32_RS08255	1902387..1903265	+	879	WP_058361060.1	helicase RepA family protein	-
ISN32_RS08260	1903237..1903626	+	390	Protein_1632	replication protein C	-
ISN32_RS08265	1903594..1903962	-	369	WP_058361061.1	conjugal transfer transcriptional regulator TraJ	-
ISN32_RS08270	1903965..1904249	-	285	WP_058361062.1	hypothetical protein	-
ISN32_RS08275	1904261..1904503	-	243	WP_033981742.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
ISN32_RS08280	1904617..1906017	-	1401	WP_058361063.1	P-type conjugative transfer protein TrbL	-
ISN32_RS08285	1906019..1906177	-	159	WP_153476784.1	hypothetical protein	-
ISN32_RS08290	1906188..1906958	-	771	WP_206710575.1	P-type conjugative transfer protein TrbJ	-
ISN32_RS08295	1907115..1907534	-	420	WP_058361064.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISN32_RS08300	1907531..1907785	-	255	WP_003473422.1	hypothetical protein	Antitoxin
ISN32_RS08305	1908197..1908478	-	282	WP_058361065.1	TraK family protein	-
ISN32_RS08310	1909095..1909595	-	501	Protein_1642	replication protein C	-
ISN32_RS08320	1911283..1912248	+	966	WP_065898826.1	IS1595 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1894468..1911086	16618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14945.19 Da
• Isoelectric Point: 4.9167

>T179954 WP_058361064.1 NZ_CP064003:c1907534-1907115 [Xanthomonas translucens pv. translucens]
MIVLDTNVVSEAMKPEPDPAVRAWLNEQVAETLYLSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFSERVLMFDTE
AARNYAELAVKARIAGKGFPTPDGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHR

Nucleotide

• Length: 420 bp

>T179954 NZ_CP084900:c3772598-3772221 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9507.88 Da
• Isoelectric Point: 7.2071

>AT179954 WP_003473422.1 NZ_CP064003:c1907785-1907531 [Xanthomonas translucens pv. translucens]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVREILESAVKSQKRIRMGDALAELGRRVGLTNDDFAVLDQVRDKVPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT179954 NZ_CP084900:c3773056-3772688 [Escherichia coli]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCATTTGGACCAGGTCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGGTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2S7EM03