Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4610950..4611686 Replicon chromosome
Accession NZ_CP063992
Organism Klebsiella pneumoniae strain M164-1

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag HXS86_RS22460 Protein ID WP_032433360.1
Coordinates 4610950..4611432 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HXS86_RS22465 Protein ID WP_003026799.1
Coordinates 4611420..4611686 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS86_RS22440 (4607499) 4607499..4608239 + 741 WP_050597964.1 molecular chaperone -
HXS86_RS22445 (4608243) 4608243..4608821 + 579 WP_032432061.1 type 1 fimbrial protein -
HXS86_RS22450 (4608833) 4608833..4609777 + 945 WP_077254249.1 fimbrial protein -
HXS86_RS22455 (4609990) 4609990..4610589 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
HXS86_RS22460 (4610950) 4610950..4611432 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
HXS86_RS22465 (4611420) 4611420..4611686 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HXS86_RS22470 (4611999) 4611999..4612562 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
HXS86_RS22475 (4612572) 4612572..4613204 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
HXS86_RS22480 (4613222) 4613222..4614226 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
HXS86_RS22485 (4614210) 4614210..4614872 - 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
HXS86_RS22490 (4614901) 4614901..4616040 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4600007..4643741 43734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T179934 WP_032433360.1 NZ_CP063992:c4611432-4610950 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T179934 NZ_CP084899:3945348-3945455 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT179934 WP_003026799.1 NZ_CP063992:c4611686-4611420 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT179934 NZ_CP084899:c3945299-3945233 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References