Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2143840..2144459 Replicon chromosome
Accession NZ_CP063992
Organism Klebsiella pneumoniae strain M164-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HXS86_RS10385 Protein ID WP_002892050.1
Coordinates 2143840..2144058 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HXS86_RS10390 Protein ID WP_002892066.1
Coordinates 2144085..2144459 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS86_RS10350 (2139883) 2139883..2140146 + 264 WP_032432663.1 type B 50S ribosomal protein L31 -
HXS86_RS10355 (2140146) 2140146..2140286 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HXS86_RS10360 (2140283) 2140283..2140981 - 699 WP_023287311.1 GNAT family protein -
HXS86_RS10365 (2141082) 2141082..2142533 + 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
HXS86_RS10370 (2142508) 2142508..2142978 - 471 WP_020802585.1 YlaC family protein -
HXS86_RS10375 (2142999) 2142999..2143139 + 141 WP_004147370.1 hypothetical protein -
HXS86_RS10380 (2143111) 2143111..2143677 - 567 WP_002892030.1 maltose O-acetyltransferase -
HXS86_RS10385 (2143840) 2143840..2144058 - 219 WP_002892050.1 HHA domain-containing protein Toxin
HXS86_RS10390 (2144085) 2144085..2144459 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HXS86_RS10395 (2144945) 2144945..2148091 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HXS86_RS10400 (2148114) 2148114..2149307 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179924 WP_002892050.1 NZ_CP063992:c2144058-2143840 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179924 NZ_CP084899:2082536-2082910 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT179924 WP_002892066.1 NZ_CP063992:c2144459-2144085 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179924 NZ_CP084899:2082079-2082447 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References