Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1515993..1516803 Replicon chromosome
Accession NZ_CP063992
Organism Klebsiella pneumoniae strain M164-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag HXS86_RS07395 Protein ID WP_002887280.1
Coordinates 1516270..1516803 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HXS86_RS07390 Protein ID WP_002887278.1
Coordinates 1515993..1516259 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS86_RS07370 (1511903) 1511903..1512208 - 306 WP_032432083.1 cation efflux system protein CusF -
HXS86_RS07375 (1512226) 1512226..1513611 - 1386 WP_032431458.1 efflux transporter outer membrane subunit -
HXS86_RS07380 (1513784) 1513784..1514467 + 684 WP_032105399.1 copper response regulator transcription factor CusR -
HXS86_RS07385 (1514457) 1514457..1515890 + 1434 WP_023288193.1 Cu(+)/Ag(+) sensor histidine kinase -
HXS86_RS07390 (1515993) 1515993..1516259 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HXS86_RS07395 (1516270) 1516270..1516803 + 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
HXS86_RS07400 (1516850) 1516850..1517971 - 1122 WP_009309849.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T179923 WP_002887280.1 NZ_CP063992:1516270-1516803 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T179923 NZ_CP084899:c1927751-1927648 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT179923 WP_002887278.1 NZ_CP063992:1515993-1516259 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT179923 NZ_CP084899:1927610-1927881 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References