Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 921348..921973 Replicon chromosome
Accession NZ_CP063992
Organism Klebsiella pneumoniae strain M164-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HXS86_RS04595 Protein ID WP_002882817.1
Coordinates 921590..921973 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HXS86_RS04590 Protein ID WP_004150355.1
Coordinates 921348..921590 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS86_RS04565 (917339) 917339..917938 + 600 WP_004151865.1 glucose-1-phosphatase -
HXS86_RS04570 (917932) 917932..918792 + 861 WP_004146232.1 virulence factor BrkB family protein -
HXS86_RS04575 (918789) 918789..919226 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HXS86_RS04580 (919271) 919271..920212 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HXS86_RS04585 (920226) 920226..921143 - 918 WP_021466676.1 alpha/beta hydrolase -
HXS86_RS04590 (921348) 921348..921590 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HXS86_RS04595 (921590) 921590..921973 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HXS86_RS04600 (922147) 922147..923076 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HXS86_RS04605 (923073) 923073..923708 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HXS86_RS04610 (923705) 923705..924607 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179920 WP_002882817.1 NZ_CP063992:921590-921973 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179920 NZ_CP084899:1511610-1511786 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179920 WP_004150355.1 NZ_CP063992:921348-921590 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179920 NZ_CP084899:1511832-1512248 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References