Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 2837..3359 Replicon plasmid p25DN-A
Accession NZ_CP063984
Organism Escherichia coli strain 25DN

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag IR210_RS24540 Protein ID WP_000220560.1
Coordinates 2837..3118 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag IR210_RS24545 Protein ID WP_000121743.1
Coordinates 3108..3359 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IR210_RS24500 125..478 - 354 Protein_1 H-NS histone family protein -
IR210_RS24505 709..981 - 273 WP_000160399.1 hypothetical protein -
IR210_RS24510 994..1143 - 150 WP_000003880.1 hypothetical protein -
IR210_RS24515 1213..1408 - 196 Protein_4 hypothetical protein -
IR210_RS24520 1451..1780 - 330 WP_000866648.1 hypothetical protein -
IR210_RS24525 1873..2088 - 216 WP_001180116.1 hypothetical protein -
IR210_RS24530 2078..2323 - 246 WP_000356548.1 hypothetical protein -
IR210_RS24535 2368..2691 - 324 WP_001181903.1 hypothetical protein -
IR210_RS24540 2837..3118 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IR210_RS24545 3108..3359 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
IR210_RS24550 4581..5420 + 840 WP_001546350.1 replication initiation protein -
IR210_RS24555 5441..6283 + 843 WP_194446392.1 replication initiation protein -
IR210_RS24560 6287..6730 + 444 WP_001074379.1 hypothetical protein -
IR210_RS24565 6801..7226 + 426 WP_000202958.1 hypothetical protein -
IR210_RS24570 7223..8200 - 978 WP_001269914.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..24185 24185


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T179916 WP_000220560.1 NZ_CP063984:c3118-2837 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T179916 NZ_CP084899:c1270016-1269909 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT179916 WP_000121743.1 NZ_CP063984:c3359-3108 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT179916 NZ_CP084899:1270064-1270130 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References