Detailed information of TA system TA179911

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4337412..4337988
• Replicon: chromosome
• Accession: NZ_CP063983
• Organism: Escherichia coli strain 25DN

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A061KXE4
• Locus tag: IR210_RS20785
• Protein ID: WP_001295743.1
• Coordinates: 4337701..4337988 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: IR210_RS20780
• Protein ID: WP_194446059.1
• Coordinates: 4337412..4337714 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IR210_RS20765	4334008..4336158	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
IR210_RS20770	4336208..4336411	+	204	WP_000467859.1	YbdD/YjiX family protein	-
IR210_RS20775	4336422..4337378	+	957	WP_001295745.1	GTPase	-
IR210_RS20780	4337412..4337714	-	303	WP_194446059.1	BrnA antitoxin family protein	Antitoxin
IR210_RS20785	4337701..4337988	-	288	WP_001295743.1	BrnT family toxin	Toxin
IR210_RS20790	4338185..4339351	+	1167	WP_000800831.1	restriction endonuclease	-
IR210_RS20795	4339415..4341034	+	1620	WP_001029745.1	type I restriction-modification system subunit M	-
IR210_RS20800	4341024..4342328	+	1305	WP_194446060.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11156.62 Da
• Isoelectric Point: 7.4697

>T179911 WP_001295743.1 NZ_CP063983:c4337988-4337701 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T179911 NZ_CP084898:c4590147-4589806 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11651.19 Da
• Isoelectric Point: 9.8218

>AT179911 WP_194446059.1 NZ_CP063983:c4337714-4337412 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRRGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT179911 NZ_CP084898:4590142-4590218 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A061KXE4