Detailed information of TA system TA179905

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3092797..3093631
• Replicon: chromosome
• Accession: NZ_CP063983
• Organism: Escherichia coli strain 25DN

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: IR210_RS14950
• Protein ID: WP_000854690.1
• Coordinates: 3092797..3093174 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IR210_RS14955
• Protein ID: WP_001546171.1
• Coordinates: 3093263..3093631 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IR210_RS14915	3088389..3088943	-	555	WP_001001909.1	molecular chaperone YcdY	-
IR210_RS14920	3088967..3089704	-	738	WP_000283664.1	zinc-binding phosphatase	-
IR210_RS14925	3089759..3090697	-	939	WP_000351287.1	glyoxylate/hydroxypyruvate reductase GhrA	-
IR210_RS14935	3091184..3092011	-	828	WP_001304477.1	DUF4942 domain-containing protein	-
IR210_RS14940	3092096..3092293	-	198	WP_000772027.1	DUF957 domain-containing protein	-
IR210_RS14945	3092313..3092800	-	488	Protein_2935	hypothetical protein	-
IR210_RS14950	3092797..3093174	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
IR210_RS14955	3093263..3093631	-	369	WP_001546171.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IR210_RS14960	3093681..3094326	-	646	Protein_2938	antitoxin of toxin-antitoxin stability system	-
IR210_RS14965	3094345..3094566	-	222	WP_000692329.1	DUF987 domain-containing protein	-
IR210_RS14970	3094629..3095105	-	477	WP_001537421.1	RadC family protein	-
IR210_RS14975	3095121..3095594	-	474	WP_000855076.1	antirestriction protein	-
IR210_RS14980	3095857..3096678	-	822	WP_001234710.1	DUF945 domain-containing protein	-
IR210_RS14985	3096759..3096970	+	212	Protein_2943	hypothetical protein	-
IR210_RS14990	3097089..3097544	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T179905 WP_000854690.1 NZ_CP063983:c3093174-3092797 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T179905 NZ_CP084898:c3240530-3240216 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13745.37 Da
• Isoelectric Point: 4.6220

>AT179905 WP_001546171.1 NZ_CP063983:c3093631-3093263 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT179905 NZ_CP084898:c3240219-3239803 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: S1PLF5