Detailed information of TA system TA179893

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1931577..1932408
• Replicon: chromosome
• Accession: NZ_CP063983
• Organism: Escherichia coli strain 25DN

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: IR210_RS09055
• Protein ID: WP_000854814.1
• Coordinates: 1931577..1931951 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1PWQ3
• Locus tag: IR210_RS09060
• Protein ID: WP_001285586.1
• Coordinates: 1932040..1932408 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IR210_RS09015	1926982..1928201	+	1220	Protein_1764	ISL3-like element ISEc53 family transposase	-
IR210_RS09020	1928258..1928731	+	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
IR210_RS09025	1928929..1929987	+	1059	WP_001200890.1	FUSC family protein	-
IR210_RS09030	1930159..1930488	+	330	WP_000450409.1	DUF496 family protein	-
IR210_RS09035	1930589..1930771	-	183	WP_001016348.1	hypothetical protein	-
IR210_RS09040	1930798..1930971	+	174	WP_032082634.1	transposase domain-containing protein	-
IR210_RS09045	1931260..1931373	-	114	WP_001161660.1	DUF957 domain-containing protein	-
IR210_RS09050	1931386..1931580	-	195	WP_000988601.1	hypothetical protein	-
IR210_RS09055	1931577..1931951	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
IR210_RS09060	1932040..1932408	-	369	WP_001285586.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
IR210_RS09065	1932482..1932703	-	222	WP_000692323.1	DUF987 domain-containing protein	-
IR210_RS09070	1932772..1933248	-	477	WP_001351157.1	RadC family protein	-
IR210_RS09075	1933264..1933752	-	489	WP_194446332.1	antirestriction protein	-
IR210_RS09080	1933843..1934664	-	822	WP_001234569.1	DUF945 domain-containing protein	-
IR210_RS09085	1934885..1935295	-	411	WP_000846704.1	hypothetical protein	-
IR210_RS09090	1935311..1935988	-	678	WP_194446377.1	hypothetical protein	-
IR210_RS09095	1936124..1937194	-	1071	WP_000102631.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T179893 WP_000854814.1 NZ_CP063983:c1931951-1931577 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T179893 NZ_CP084898:1511610-1511786 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13563.41 Da
• Isoelectric Point: 5.0468

>AT179893 WP_001285586.1 NZ_CP063983:c1932408-1932040 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT179893 NZ_CP084898:1511832-1512248 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: S1PWQ3