Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 997556..998210 Replicon chromosome
Accession NZ_CP063983
Organism Escherichia coli strain 25DN

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag IR210_RS04855 Protein ID WP_000244781.1
Coordinates 997803..998210 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IR210_RS04850 Protein ID WP_000354046.1
Coordinates 997556..997822 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IR210_RS04830 993644..995077 - 1434 WP_194446259.1 6-phospho-beta-glucosidase BglA -
IR210_RS04835 995122..995433 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
IR210_RS04840 995597..996256 + 660 WP_000250274.1 hemolysin III family protein -
IR210_RS04845 996333..997313 - 981 WP_000886079.1 tRNA-modifying protein YgfZ -
IR210_RS04850 997556..997822 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IR210_RS04855 997803..998210 + 408 WP_000244781.1 protein YgfX Toxin
IR210_RS04860 998250..998771 - 522 WP_001055874.1 flavodoxin FldB -
IR210_RS04865 998883..999779 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IR210_RS04870 999804..1000514 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IR210_RS04875 1000520..1002253 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T179890 WP_000244781.1 NZ_CP063983:997803-998210 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T179890 NZ_CP084898:c1270551-1270444 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT179890 WP_000354046.1 NZ_CP063983:997556-997822 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT179890 NZ_CP084898:1270598-1270664 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References