Detailed information of TA system TA179868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 592601..593172
• Replicon: chromosome
• Accession: NZ_CP063980
• Organism: Enterococcus faecalis strain AR_0780

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ISN59_RS02850
• Protein ID: WP_002354774.1
• Coordinates: 592831..593172 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S4CGQ1
• Locus tag: ISN59_RS02845
• Protein ID: WP_002367500.1
• Coordinates: 592601..592831 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISN59_RS02825 (588929)	588929..590224	+	1296	WP_002410675.1	ATP-binding protein	-
ISN59_RS02830 (590233)	590233..590865	+	633	WP_002358972.1	RloB family protein	-
ISN59_RS02835 (590932)	590932..591924	-	993	WP_002358973.1	biotin--[acetyl-CoA-carboxylase] ligase	-
ISN59_RS02840 (592063)	592063..592278	+	216	WP_002354768.1	zinc ribbon domain-containing protein	-
ISN59_RS02845 (592601)	592601..592831	+	231	WP_002367500.1	hypothetical protein	Antitoxin
ISN59_RS02850 (592831)	592831..593172	+	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ISN59_RS02855 (593543)	593543..597157	+	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T179868 WP_002354774.1 NZ_CP063980:592831-593172 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T179868 NZ_CP084897:c1648978-1648691 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8704.69 Da
• Isoelectric Point: 4.3313

>AT179868 WP_002367500.1 NZ_CP063980:592601-592831 [Enterococcus faecalis]
MLTTKSRRQGNSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT179868 NZ_CP084897:c1649217-1648978 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: S4CGQ1