Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1437809..1438451 Replicon chromosome
Accession NZ_CP063975
Organism Bacillus cereus strain N435-1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag IP614_RS07535 Protein ID WP_000635965.1
Coordinates 1438101..1438451 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag IP614_RS07530 Protein ID WP_000004570.1
Coordinates 1437809..1438096 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IP614_RS07505 1433126..1434088 + 963 WP_000961161.1 UV DNA damage repair endonuclease UvsE -
IP614_RS07510 1434081..1434653 - 573 WP_074625534.1 rhomboid family intramembrane serine protease -
IP614_RS07515 1434747..1435106 + 360 WP_000583417.1 holo-ACP synthase -
IP614_RS07520 1435263..1436213 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
IP614_RS07525 1436331..1437500 + 1170 WP_242785340.1 alanine racemase -
IP614_RS07530 1437809..1438096 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
IP614_RS07535 1438101..1438451 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IP614_RS07540 1438519..1440687 + 2169 WP_044306890.1 Tex family protein -
IP614_RS07545 1440745..1440861 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
IP614_RS07550 1441057..1441515 + 459 WP_047385515.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T179861 WP_000635965.1 NZ_CP063975:1438101-1438451 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T179861 NZ_CP084897:c479532-479314 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT179861 WP_000004570.1 NZ_CP063975:1437809-1438096 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT179861 NZ_CP084897:c479932-479558 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References