Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4502232..4502818 Replicon chromosome
Accession NZ_CP063955
Organism Klebsiella aerogenes strain S148-1

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag IOP81_RS21355 Protein ID WP_002920800.1
Coordinates 4502232..4502600 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A6B2JAG3
Locus tag IOP81_RS21360 Protein ID WP_046883659.1
Coordinates 4502597..4502818 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOP81_RS21335 4497735..4498805 - 1071 WP_015369330.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
IOP81_RS21340 4498807..4499652 - 846 WP_015369329.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
IOP81_RS21345 4499649..4500536 - 888 WP_015369328.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
IOP81_RS21350 4500643..4501959 - 1317 WP_242814272.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
IOP81_RS21355 4502232..4502600 - 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IOP81_RS21360 4502597..4502818 - 222 WP_046883659.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IOP81_RS21365 4502982..4503695 - 714 WP_086538103.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
IOP81_RS21370 4503713..4504480 - 768 WP_015369325.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
IOP81_RS21375 4504477..4505754 - 1278 WP_015369324.1 branched chain amino acid ABC transporter permease LivM -
IOP81_RS21380 4505751..4506677 - 927 WP_015369323.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4496998..4504480 7482


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T179858 WP_002920800.1 NZ_CP063955:c4502600-4502232 [Klebsiella aerogenes]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T179858 NZ_CP084897:c246239-245961 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8093.12 Da        Isoelectric Point: 5.0637

>AT179858 WP_046883659.1 NZ_CP063955:c4502818-4502597 [Klebsiella aerogenes]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGGEIRELADK

Download         Length: 222 bp

>AT179858 NZ_CP084897:c246502-246242 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A6B2JAG3

References