Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4946266..4946891 Replicon chromosome
Accession NZ_CP063954
Organism Klebsiella variicola strain S129-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag IOD50_RS23785 Protein ID WP_008807903.1
Coordinates 4946508..4946891 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IOD50_RS23780 Protein ID WP_004150355.1
Coordinates 4946266..4946508 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD50_RS23755 4942257..4942856 + 600 WP_008807908.1 glucose-1-phosphatase -
IOD50_RS23760 4942850..4943710 + 861 WP_008807907.1 virulence factor BrkB family protein -
IOD50_RS23765 4943707..4944144 + 438 WP_012543288.1 D-tyrosyl-tRNA(Tyr) deacylase -
IOD50_RS23770 4944189..4945130 + 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
IOD50_RS23775 4945144..4946061 - 918 WP_016162034.1 alpha/beta hydrolase -
IOD50_RS23780 4946266..4946508 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IOD50_RS23785 4946508..4946891 + 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
IOD50_RS23790 4947064..4947993 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IOD50_RS23795 4947990..4948625 - 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
IOD50_RS23800 4948622..4949524 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179844 WP_008807903.1 NZ_CP063954:4946508-4946891 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T179844 NZ_CP084896:c2094432-2094178 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179844 WP_004150355.1 NZ_CP063954:4946266-4946508 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179844 NZ_CP084896:c2094680-2094429 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References