Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4590418..4591049 Replicon chromosome
Accession NZ_CP063954
Organism Klebsiella variicola strain S129-1

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag IOD50_RS22180 Protein ID WP_044650325.1
Coordinates 4590774..4591049 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag IOD50_RS22175 Protein ID WP_004181489.1
Coordinates 4590418..4590777 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD50_RS22150 4586675..4587619 + 945 WP_008806948.1 nickel ABC transporter permease subunit NikB -
IOD50_RS22155 4587616..4588449 + 834 WP_194394897.1 nickel ABC transporter permease subunit NikC -
IOD50_RS22160 4588449..4589213 + 765 WP_023340796.1 nickel import ATP-binding protein NikD -
IOD50_RS22165 4589210..4590001 + 792 WP_012540373.1 nickel import ATP-binding protein NikE -
IOD50_RS22170 4589989..4590390 + 402 WP_194394896.1 nickel-responsive transcriptional regulator NikR -
IOD50_RS22175 4590418..4590777 - 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IOD50_RS22180 4590774..4591049 - 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
IOD50_RS22185 4591446..4592459 + 1014 WP_012540371.1 magnesium transporter -
IOD50_RS22190 4592499..4593692 - 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
IOD50_RS22195 4593922..4595418 + 1497 WP_008806938.1 inorganic phosphate transporter PitA -
IOD50_RS22200 4595478..4595813 - 336 WP_002921203.1 universal stress protein UspB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T179843 WP_044650325.1 NZ_CP063954:c4591049-4590774 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T179843 NZ_CP084896:2082536-2082910 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT179843 WP_004181489.1 NZ_CP063954:c4590777-4590418 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT179843 NZ_CP084896:2082079-2082447 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References