Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3074142..3074732 Replicon chromosome
Accession NZ_CP063954
Organism Klebsiella variicola strain S129-1

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag IOD50_RS14830 Protein ID WP_008804165.1
Coordinates 3074142..3074474 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag IOD50_RS14835 Protein ID WP_012541132.1
Coordinates 3074475..3074732 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD50_RS14805 3070182..3070427 + 246 WP_008804171.1 signal transduction protein PmrD -
IOD50_RS14810 3070556..3072010 + 1455 WP_064160537.1 AMP nucleosidase -
IOD50_RS14820 3072383..3073819 - 1437 WP_042948427.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
IOD50_RS14830 3074142..3074474 - 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IOD50_RS14835 3074475..3074732 - 258 WP_012541132.1 antitoxin Antitoxin
IOD50_RS14840 3075258..3076358 + 1101 WP_080928497.1 phosphotransferase -
IOD50_RS14845 3076355..3077326 - 972 WP_008804156.1 sensor domain-containing diguanylate cyclase -
IOD50_RS14850 3077532..3078368 - 837 WP_008804155.1 alpha/beta hydrolase -
IOD50_RS14855 3078545..3079120 - 576 WP_016161434.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T179839 WP_008804165.1 NZ_CP063954:c3074474-3074142 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T179839 NZ_CP084896:1511610-1511786 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT179839 WP_012541132.1 NZ_CP063954:c3074732-3074475 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179839 NZ_CP084896:1511832-1512248 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References